INDUCTION OF MICRONUCLEI IN MICE BONE MARROW CELLS BY COBALT AND COPPER CHLORIDES
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Date
2013
Journal Title
Journal ISSN
Volume Title
Publisher
Polska Akad Nauk, Polish Acad Sciences, Inst Environ Eng Pas
Access Rights
info:eu-repo/semantics/openAccess
Abstract
The aim of our research was to investigate the genotoxic effects of cobalt chloride and copper chloride in mouse bone marrow cells using the micronucleus (MN) assay. The three different concentrations of cobalt chloride (11.2, 22.5 and 45 mg kg(-1)) and copper chloride (1.17, 2.35 and 4.70 mg kg(-1)) were injected intraperitoneally to mice for 24 and 48 hours. It was observed that both of these heavy metals induced a significant increase in frequency of micronucleated polychromatic erythrocytes (MNPCE) at different concentrations in mice for 24 and 48 hours when compared with the control. Furthermore, the significant reduction for the polychromatic erythrocyte/normochromatic erythrocyte (PCE/NCE) ratio which is indicative of bone marrow cytotoxicity was observed in bone marrow cells which were treated with copper chloride at all concentrations for 24 and 48 hours. No reduction of the PCE/NCE ratio was observed both 24 and 48 hours after all the doses of cobalt chloride tested as compared to the negative control. These results lead us to the conclusion that copper chloride may have genotoxic and cytotoxic properties due to induction in the frequency of MN and a reduction in PCE/NCE ratio in bone marrow cells of mice, whereas cobalt chloride induced only genotoxic effect in mice bone marrow.
Description
WOS: 000316162100007
Keywords
Cobalt chloride, copper chloride, heavy metal, mice, micronucleus assay
Journal or Series
Archives Of Environmental Protection
WoS Q Value
Q4
Scopus Q Value
N/A
Volume
39
Issue
1
