Genome-wide identification and expression analysis of sulphate transporter (SULTR) genes under sulfur deficiency in Brachypodium distachyon
Yükleniyor...
Dosyalar
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer India
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Sulphur is an important mineral element for plant growth and development. It involves in a number of metabolic processes with crucial functions. This study has performed a genome-wide analysis of sulfate transporter (SULTR) genes in Brachypodium distachyon. Ten putative SULTR genes were identified in Brachypodium genome. BdSULTR genes included 6-17 exons encoding a protein of 647-693 residues with basic nature. BdSULTR proteins included both sulfate_transp (PF00916) and STAS (PF01740) domains. BdSULTRs were classified into 4 groups based on the phylogenetic distribution. Promoter regions of all BdSULTR genes, except for BdSULTR3;3 and 3;5 included the SURECOREATSULTR11 elements. A considerable structural overlap was identified between superimposed SULTR1;3 and 3;1 proteins, indicating that SULTR1 members may also involve in plant stress response/tolerance like SULTR3 members. Microarray and RNA-Seq analyses also revealed the differential expression of SULTR 1 and 3 genes under different biotic/abiotic stresses. Protein-protein interaction partners of BdSULTRs were mainly related with adenylyl-sulfate kinases, 5'-adenylylsulfate reductases, ATP sulfurylases, and acyl carrier proteins. Moreover, expression profiles of identified BdSULTR genes under S-deficiency were analyzed using RT-qPCR. It was revealed that BdSULTR1;1 and 3;1 are highly expressed in plant roots as similar to tenfold and similar to fivefold, respectively, while BdSULTR2 (similar to 15-fold) and 3;1 (similar to twofold) are abundantly expressed in leaf tissues.
Açıklama
Tombuloglu, Huseyin/0000-0001-8546-2658
WOS: 000404618300002
WOS: 000404618300002
Anahtar Kelimeler
Abiotic stress, Microarray, Protein interactions, SULTR, Sulphur deficiency, SURE box
Kaynak
Journal Of Plant Biochemistry And Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
26
Sayı
3
