Ex Vivo Investigation of Bexarotene and Nicotinamide Function as a Protective Agent on Rat Synaptosomes Treated with A beta(1-42)

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dc.authoridkar, fatih/0000-0001-8356-9806
dc.authorwosidkar, fatih/AAN-4285-2021
dc.authorwosidkar, fatih/AAI-4540-2021
dc.contributor.authorHacioglu, Ceyhan
dc.contributor.authorKar, Fatih
dc.contributor.authorKanbak, Gungor
dc.date.accessioned2021-12-01T18:48:23Z
dc.date.available2021-12-01T18:48:23Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractIn this study, we were aimed to investigate the neuroprotective effects of bexarotene and nicotinamide in synaptosomes incubated with amyloid-beta (A beta). Our study consists of 2 parts, in vivo and in vitro. In the in vivo section, twenty-four Wistar albino male rats were divided into 4 groups (control, dimethyl sulfoxide (DMSO), nicotinamide and bexarotene) with six animals in each group. DMSO(1%), nicotinamide(100 mg/kg) and bexarotene(0.1 mg/kg) were administered intraperitoneally to animals in the experimental groups for seven days. In the in vitro part of our study, three different isolation methods were used to obtain the synaptosomes from the brain tissue. Total antioxidant capacity(TAS), total oxidant capacity(TOS), cleaved caspase 3(CASP3), cytochrome c(Cyt c), sirtuin 1(SIRT1), peroxisome proliferator-activated receptor gamma(PPAR gamma) and poly(ADP-ribose) polymerase-1(PARP-1) levels in the synaptosomes incubated with a concentration of 10 mu M A beta(1-42) were measured by enzyme-linked immunosorbent assay method. Biochemical analysis and histopathological examinations in serum and brain samples showed that DMSO, nicotinamide and bexarotene treatments did not cause any damage to the rat brain tissue. We found that in vitro A beta(1-42) administration decreased TAS, SIRT1 and PPAR gamma levels in synaptosomes while increasing TOS, CASP3, Cyt c, and PARP1 levels. Nicotinamide treatment suppressed oxidative stress and apoptosis by supporting antioxidant capacity and increased PPAR gamma through SIRT1 activation, causing PARP1 to decrease. On the other hand, bexarotene caused a moderate increase in SIRT1 levels with PPAR gamma activation. Consequently, we found that nicotinamide can be more effective than bexarotene in AD pathogenesis by regulating mitochondrial functions in synaptosomes.en_US
dc.description.sponsorshipEskisehir Osmangazi University Scientific Research CommissionEskisehir Osmangazi University [201811D21]en_US
dc.description.sponsorshipThis study was supported by Eskisehir Osmangazi University Scientific Research Commission with grant number 201811D21.en_US
dc.identifier.doi10.1007/s11064-020-03216-7
dc.identifier.endpage818en_US
dc.identifier.issn0364-3190
dc.identifier.issn1573-6903
dc.identifier.issue4en_US
dc.identifier.pmid33428094en_US
dc.identifier.scopus2-s2.0-85099087207en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage804en_US
dc.identifier.urihttps://doi.org/10.1007/s11064-020-03216-7
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10522
dc.identifier.volume46en_US
dc.identifier.wosWOS:000607028800002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer/Plenum Publishersen_US
dc.relation.ispartofNeurochemical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlzheimer's diseaseen_US
dc.subjectAntioxidanten_US
dc.subjectApoptosisen_US
dc.subjectBexaroteneen_US
dc.subjectNicotinamideen_US
dc.subjectOxidative stressen_US
dc.subjectPARP1en_US
dc.subjectPpar gammaen_US
dc.subjectSIRT1en_US
dc.subjectSynaptosomesen_US
dc.subjectAmyloid-Betaen_US
dc.subjectAlzheimers-Diseaseen_US
dc.subjectOxidative Stressen_US
dc.subjectSirt1en_US
dc.subjectTauen_US
dc.subjectMetabolismen_US
dc.subjectActivationen_US
dc.subjectNad(+)en_US
dc.subjectPoly(Adp-Ribose)en_US
dc.subjectInhibitionen_US
dc.titleEx Vivo Investigation of Bexarotene and Nicotinamide Function as a Protective Agent on Rat Synaptosomes Treated with A beta(1-42)en_US
dc.typeArticleen_US

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