Determination of histological, immunohistochemical and biochemical effects of acute and chronic grayanotoxin III administration in different doses in rats

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dc.contributor.authorDoğanyiğit, Züleyha
dc.contributor.authorSilici, Sibel
dc.contributor.authorDemirtaş, Abdullah
dc.contributor.authorKaya, Ertuğrul
dc.contributor.authorKaymak, Emin
dc.date.accessioned2020-05-01T09:11:20Z
dc.date.available2020-05-01T09:11:20Z
dc.date.issued2019
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.descriptionDemirtas, Abdullah/0000-0001-9102-5518en_US
dc.descriptionWOS: 000455814700030en_US
dc.descriptionPubMed: 30426365en_US
dc.description.abstractGrayanotoxin (GTX)-III is a Na-channel neurotoxin. Grayanotoxins can be found in the nectar, pollen, and other plant parts of the Rhododendron genus plants from the Ericaceae family. It is widely believed that honey produced from these plants, which are concentrated in the Black Sea region, is traditionally characterized as enhancing sexual performance. It is thought that the effective factor is dose for this compound, which has both beneficial and toxic effects reported. Therefore, it is aimed to evaluate the histological, immunohistochemical, and biochemical effects of acute and chronic impact of GTX-III in different doses on testes tissue in this study. For this purpose, 100 Sprague-Dawley male rats were divided into 5 separate groups for acute and chronic research. While dose groups were (control, 0.1, 0.2, 0.4, ve 0.8g/kg/bw) for experimental groups, a single dose (i.p.) was administered for acute impact whereas the same doses were administered daily for 3weeks to assess chronic effect. At the end of the experiment, Johnsen testicular biopsy scoring was performed on testicular tissue samples, seminiferous tubule diameters were measured, and apoptotic cells were evaluated by TUNEL method. Testosterone, LH, and FSH levels were measured by ELISA method in serum and tissue specimens. It was found that Johnsen score of acute doses was significantly lower than the control group, and the diameter of the seminiferous tubules decreased significantly in acute and chronic dose-administered groups compared to the control. Hemorrhage, epithelial shedding, irregularity in seminiferous epithelium, and vacuolization were observed in acute and chronic dose-administered groups, and increase in apoptotic cells was determined. Hormone levels varied depending on the dose. In conclusion, it was found that dose-dependent acute and chronic effects of GTX-III are different, and this factor should be taken into account in studies to be carried out due to the adverse effects of high doses.en_US
dc.description.sponsorshipBozok University Project Coordination Application and Research CenterBozok University [6602c-TF/17-60]en_US
dc.description.sponsorshipThis work was supported by Bozok University Project Coordination Application and Research Center (6602c-TF/17-60).en_US
dc.identifier.doi10.1007/s11356-018-3700-9en_US
dc.identifier.endpage1335en_US
dc.identifier.issn0944-1344
dc.identifier.issn1614-7499
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1323en_US
dc.identifier.urihttps://doi.org/10.1007/s11356-018-3700-9
dc.identifier.urihttps://hdl.handle.net/20.500.12684/5517
dc.identifier.volume26en_US
dc.identifier.wosWOS:000455814700030en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofEnvironmental Science And Pollution Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGrayanotoxinen_US
dc.subjectEricaceaeen_US
dc.subjectSexual hormoneen_US
dc.subjectHistologyen_US
dc.titleDetermination of histological, immunohistochemical and biochemical effects of acute and chronic grayanotoxin III administration in different doses in ratsen_US
dc.typeArticleen_US

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