Qualitative enzymatic detection of organophosphate and carbamate insecticides

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Tarih

2020

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Taylor & Francis Inc

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

In this study, new polymers containing amides (TrisPS-Ntaa, andTrisPS-Ntaa-Fc) were synthesized by condensation reaction for qualitative identification of insecticides. The synthesized polymers, including amides were investigated by infrared spectroscopy (IR), scanning electron microscopy-energy dispersion X- ray spectrometry (SEM-EDX), and gel permeation chromatography (GPC). Then, acetylcholinesterase enzyme (AChE) was covalently immobilized on these polymers to improve properties (including activity, reusability, and storage stability). Accordingly, organophosphate (malathion, acephate, chlorpyrifos methyl) and carbamate (carbofuran, methiocarb, methomyl), which are used to prevent harmful organisms in some agricultural products were enzymatically determined based on their inhibitory activity on AChE.

Açıklama

Anahtar Kelimeler

Acetylcholinesterase, carbamate, organophosphate, polymers containing azomethine, tris(2-aminoethyl)amine polymer-bound, Acetylcholinesterase, Immobilization, Pesticides, Biosensors, Selectivity, Stability

Kaynak

Journal Of Environmental Science And Health Part B-Pesticides Food Contaminants And Agricultural Wastes

WoS Q Değeri

Q3

Scopus Q Değeri

Q2

Cilt

55

Sayı

11

Künye