Exploring the antibiofilm effects on Escherichia coli biofilm associated with colon cancer and anticancer activities on HCT116 cell line of bee products

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dc.authoridAKKOYUNLU, Aysegul/0000-0002-3663-7412en_US
dc.authorscopusid57934358000en_US
dc.authorscopusid25229718700en_US
dc.contributor.authorAkkoyunlu, Aysegul
dc.contributor.authorDülger, Görkem
dc.date.accessioned2024-08-23T16:04:28Z
dc.date.available2024-08-23T16:04:28Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractThe association between dysbiotic microbiota biofilm and colon cancer has recently begun to attract attention. In the study, the apitherapeutic effects of bee products (honey, bee venom, royal jelly, pollen, perga and propolis) obtained from the endemic Y & imath;& gbreve;& imath;lca ecotype of Apis mellifera anatoliaca were investigated. Antibiofilm activity were performed by microplate assay using crystal violet staining to measure adherent biofilm biomass of Escherichia coli capable of forming biofilms. Bee venom showed the highest inhibition effect (73.98%) at 50% concentration. Honey, perga and royal jelly reduced biofilm formation by >50% at all concentrations. The antiproliferation effect on the HCT116 colon cancer cell line was investigated with the water-soluble tetrazolium salt-1 assay. After 48 h of honey application at 50% concentration, cell proliferation decreased by 86.51%. The high cytotoxic effects of royal jelly and bee venom are also remarkable. Additionally, apoptotic pathway analysis was performed by ELISA using caspase 3, 8 and 9 enzyme-linked immunosorbent assay kits. All bee products induced a higher expression of caspase 9 compared with caspase 8. Natural products that upregulate caspase proteins are promising therapeutic targets for proliferative diseases.en_US
dc.description.sponsorshipDzce Universityen_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.doi10.1080/08927014.2024.2338106
dc.identifier.endpage244en_US
dc.identifier.issn0892-7014
dc.identifier.issn1029-2454
dc.identifier.issue3-4en_US
dc.identifier.pmid38584359en_US
dc.identifier.scopus2-s2.0-85189638214en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage235en_US
dc.identifier.urihttps://doi.org/10.1080/08927014.2024.2338106
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14213
dc.identifier.volume40en_US
dc.identifier.wosWOS:001198475500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiofoulingen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHoney bee productsen_US
dc.subjectantibiofilmen_US
dc.subjectWST-1en_US
dc.subjectCaspase 3, 8, 9en_US
dc.subjectPollenen_US
dc.subjectHoneyen_US
dc.subjectGuten_US
dc.titleExploring the antibiofilm effects on Escherichia coli biofilm associated with colon cancer and anticancer activities on HCT116 cell line of bee productsen_US
dc.typeArticleen_US

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