Hydroxylated polymeric linolenic acid containing cytocompatible cryogels with antibiofilm activities
Küçük Resim Yok
Tarih
2025
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Essential oils and their acids can possess various beneficial properties, such as antioxidant, antiviral, antifungal, and antibacterial activities. Therefore, we conducted a comprehensive examination of the biological activities of HEMA-based cryogels containing hydroxylated polymeric linolenic acid (PLinoOH). First, linolenic acid underwent autoxidation for three months to yield macroperoxide linolenic acid. This macro peroxide fatty acid, distinguished by its peroxide, epoxide, and hydroperoxide functional groups, reacted with diethanolamine to synthesize hydroxylated polymeric linolenic acid (PLinoOH). The resulting PLinoOH was then utilized to prepare the HEMA-based cryogel series. These cryogels were physically and chemically characterized. The cytocompatibility of the cryogel series and its potential genotoxic effects on cellular DNA were examined using human embryonic kidney cells. The antifungal and antibacterial effects of the cryogel series were evaluated against Saccharomyces cerevisiae yeast and Staphylococcus aureus, Enterococcus faecalis, and Pseudomonas aeruginosa bacterial strains. Additionally, total sugar, uronic acid, and protein analyses were performed to understand their effects on the exopolysaccharide substance in the biofilm structure. The fabricated cryogels exhibited considerable antibiofilm effects along with their remarkable biocompatibility. They showed varying effects were observed on the structure of exopolysaccharide substance according to the bacterial species and cell wall structure. Overall, these findings highlight the potentials of the fabricated cryogels as multifunctional biomaterials for combating microbial infections, with species-specific impacts on biofilm extracellular polymeric substance (EPS) composition.
Açıklama
Anahtar Kelimeler
Cryogel, Linolenic acid, Antimicrobial activity, Biofilm, Comet assay
Kaynak
Materials Today Chemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
44
