In Vitro Effects of Cerium Vanadate (CeVO4) Derivatives on Paraoxonase (PON1) Enzyme

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dc.contributor.authorOnay, Yasin
dc.contributor.authorCelik Gul, Gulsah
dc.contributor.authorKurtulus, Figen
dc.contributor.authorKerimak-Oner, Mine Nazan
dc.contributor.authorDemirci, Tuna
dc.contributor.authorKaya, Mustafa Oguzhan
dc.date.accessioned2026-07-01T11:40:12Z
dc.date.available2026-07-01T11:40:12Z
dc.date.issued2026
dc.departmentDüzce Üniversitesi
dc.description.abstractCerium vanadate (CeVO4)-based materials have attracted attention due to their diverse physicochemical and biological properties; however, their interactions with detoxification-related enzymes remain insufficiently characterized. This study evaluated the in vitro effects of alkaline earth metal-doped CeVO4 derivatives (Be, Mg, Ca, Sr, and Ba) on human serum paraoxonase-1 (PON1) paraoxonase activity. PON1 was purified from human serum by hydrophobic interaction chromatography (Sepharose 4B-L-tyrosine-1-naphthylamine), and enzymatic activity was measured spectrophotometrically using paraoxon as the substrate. Inhibitory effects were assessed across multiple concentrations, and IC50 values were determined from concentration-response curves. All derivatives exhibited concentration-dependent inhibition of PON1 activity, with IC50 values ranging from 117 to 213 & micro;M. Mg-and Ba-doped CeVO4 derivatives demonstrated the strongest inhibition at higher doping ratios, whereas Ca-doped derivatives showed reduced inhibitory potency with increasing substitution levels. These findings indicate that dopant identity and concentration significantly influence the interaction between CeVO4 derivatives and PON1. The study provides insight into metal oxide-enzyme interactions and highlights the importance of dopant-dependent effects in evaluating the biological and potential toxicological behavior of engineered vanadate-based materials.
dc.identifier.doi10.1007/s10930-026-10330-y
dc.identifier.endpage486
dc.identifier.issn1572-3887
dc.identifier.issn1875-8355
dc.identifier.issue3
dc.identifier.pmid42050307
dc.identifier.scopus2-s2.0-105037579734
dc.identifier.scopusqualityQ3
dc.identifier.startpage479
dc.identifier.urihttps://doi.org/10.1007/s10930-026-10330-y
dc.identifier.urihttps://hdl.handle.net/20.500.12684/23680
dc.identifier.volume45
dc.identifier.wosWOS:001752563800001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofProtein Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260623
dc.subject[Keyword Not Available]
dc.titleIn Vitro Effects of Cerium Vanadate (CeVO4) Derivatives on Paraoxonase (PON1) Enzyme
dc.typeArticle

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