Escherichia coli Enumeration in a Capillary-Driven Microfluidic Chip with SERS

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dc.authoridTamer, Uğur/0000-0001-9989-6123
dc.authoridYıldırım, Ender/0000-0002-7969-2243
dc.authoridCetin, Demet/0000-0003-1186-4229
dc.authoridBoyaci, Ismail/0000-0003-1333-060X
dc.authoridSULUDERE, ZEKIYE/0000-0002-1207-5814
dc.authorwosidBoyaci, Ismail/A-5255-2013
dc.contributor.authorDoğan, Üzeyir
dc.contributor.authorSucularlı, Ferah
dc.contributor.authorYıldırım, Ender
dc.contributor.authorCetin, Demet
dc.contributor.authorSuludere, Zekiye
dc.contributor.authorBoyacı, İsmail Hakkı
dc.contributor.authorTamer, Uğur
dc.date.accessioned2023-07-26T11:51:00Z
dc.date.available2023-07-26T11:51:00Z
dc.date.issued2022
dc.departmentDÜ, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümüen_US
dc.description.abstractPathogen detection is still a challenging issue for public health, especially in food products. A selective preconcentration step is also necessary if the target pathogen concentration is very low or if the sample volume is limited in the analysis. Plate counting (24-48 h) methods should be replaced by novel biosensor systems as an alternative reliable pathogen detection technique. The usage of a capillary-driven microfluidic chip is an alternative method for pathogen detection, with the combination of surface-enhanced Raman scattering (SERS) measurements. Here, we constructed microchambers with capillary microchannels to provide nanoparticle-pathogen transportation from one chamber to the other. Escherichia coli (E. coli) was selected as a model pathogen and specific antibody-modified magnetic nanoparticles (MNPs) as a capture probe in a complex milk matrix. MNPs that captured E. coli were transferred in a capillary-driven microfluidic chip consisting of four chambers, and 4-aminothiophenol (4-ATP)-labelled gold nanorods (Au NRs) were used as the Raman probe in the capillary-driven microfluidic chip. The MNPs provided immunomagnetic (IMS) separation and preconcentration of analytes from the sample matrix and then, 4-ATP-labelled Au NRs provided an SERS response by forming sandwich immunoassay structures in the last chamber of the capillary-driven microfluidic chip. The developed SERS-based method could detect 10(1)-10(7) cfu/mL of E. coli with the total analysis time of less than 60 min. Selectivity of the developed method was also tested by using Salmonella enteritidis (S. enteritidis) and Staphylococcus aureus (S. aureus) as analytes, and very weak signals were observed.en_US
dc.description.sponsorshipGazi University [02/2018-08]en_US
dc.description.sponsorshipThis research was funded by Gazi University grant number Gazi BAP, project number 02/2018-08.en_US
dc.identifier.doi10.3390/bios12090765
dc.identifier.issn2079-6374
dc.identifier.issue9en_US
dc.identifier.pmid36140150en_US
dc.identifier.scopus2-s2.0-85138626689en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/bios12090765
dc.identifier.urihttps://hdl.handle.net/20.500.12684/12473
dc.identifier.volume12en_US
dc.identifier.wosWOS:000857676500001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorDoğan, Üzeyir
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofBiosensors-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectEscherichia Coli; Microfluidic Chip; Sers Detection; Magnetic Separation; Gold Nanorodsen_US
dc.subjectSensitive Detection; Rapid Detection; Bacteria; Plasma; Dielectrophoresis; Nanoparticles; Biosensor; O157h7; Device; Sensoren_US
dc.titleEscherichia coli Enumeration in a Capillary-Driven Microfluidic Chip with SERSen_US
dc.typeArticleen_US

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