The effects of L-NAME on DU145 human prostate cancer cell line: A cytotoxicity-based study

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dc.authoridkar, fatih/0000-0001-8356-9806
dc.authoridKacar, Sedat/0000-0002-0671-8529
dc.authoridSAHINTURK, VAROL/0000-0003-2317-3644
dc.authorwosidkar, fatih/AAN-4285-2021
dc.authorwosidhacioglu, ceyhan/ABB-5317-2020
dc.authorwosidkar, fatih/AAI-4540-2021
dc.authorwosidKacar, Sedat/I-2544-2019
dc.authorwosidSAHINTURK, VAROL/V-5195-2017
dc.contributor.authorKacar, S.
dc.contributor.authorKar, F.
dc.contributor.authorHacioglu, C.
dc.contributor.authorKanbak, G.
dc.contributor.authorSahinturk, V
dc.date.accessioned2021-12-01T18:48:08Z
dc.date.available2021-12-01T18:48:08Z
dc.date.issued2020
dc.department[Belirlenecek]en_US
dc.description.abstractOf all cancer types, prostate cancer is the second most common one with an age-standardized incidence rate of 29.3 per 100,000 men worldwide. Nitric oxide (NO) is both a radical and versatile messenger molecule involved in many physiological activities. NO was documented to be highly secreted and utilized by cancer cells. N omega-nitro-l-arginine methyl ester (L-NAME) is utilized for inhibiting NO synthase. Its worst long-term side effect is reported to be hypertension, hence less cytotoxic than chemotherapeutic agents. Herein, we carried out a cytotoxicity study on how different doses of L-NAME affect DU145 human prostate cancer cells. First, toxic doses of L-NAME were determined. Then, while antioxidant capacity was determined by glutathione and total antioxidant status, oxidative stress was evaluated by quantifying malondialdehyde, NO, and total oxidant status levels. Inflammatory effects of L-NAME were investigated by measuring tumor necrosis factor-alpha and interleukin-6 (IL-6) levels. Apoptotic effects of L-NAME were evaluated by measuring cytochrome C somatic and caspase 3 levels and by staining Bax protein. Finally, morphological analysis was performed. IC50 of L-NAME against DU145 cells was 12.2 mM. In L-NAME-treated DU145 cells, a dose-dependent increase in oxidative stress, inflammatory, and apoptotic marker proteins and decrease in antioxidant capacity were observed. While at the moderate dose of L-NAME, apoptotic changes were commonly observed, at higher doses, vacuolated and swollen cells were also recorded. We believe that the present study will encourage future studies by providing insights about dose and effects of L-NAME.en_US
dc.identifier.doi10.1177/0960327119880591
dc.identifier.endpage193en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue2en_US
dc.identifier.pmid31610702en_US
dc.identifier.scopus2-s2.0-85074325284en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage182en_US
dc.identifier.urihttps://doi.org/10.1177/0960327119880591
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10463
dc.identifier.volume39en_US
dc.identifier.wosWOS:000490671500001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectL-NAMEen_US
dc.subjectnitric oxideen_US
dc.subjectDU145 prostate cancer cellsen_US
dc.subjectoxidative stressen_US
dc.subjectinflammationen_US
dc.subjectapoptosisen_US
dc.subjectcell morphologyen_US
dc.subjectcytotoxicityen_US
dc.subjectNitric-Oxide Synthaseen_US
dc.subjectOxidative Stressen_US
dc.subjectIn-Vitroen_US
dc.subjectHypertensionen_US
dc.subjectPathwayen_US
dc.subjectProliferationen_US
dc.subjectInhibitoren_US
dc.subjectCarcinomaen_US
dc.subjectApoptosisen_US
dc.subjectGrowthen_US
dc.titleThe effects of L-NAME on DU145 human prostate cancer cell line: A cytotoxicity-based studyen_US
dc.typeArticleen_US

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