Ratiometric fluorescence detection of an anthrax biomarker with Eu3+-chelated chitosan biopolymers
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel chitosan-based ratiometric fluorescent probe incorporating an EDTA-Eu3+ complex as the sensing unit and fluorescein dye as the internal standard was designed to detect dipicolinic acid (DPA) as an anthrax bio-marker with high sensitivity and selectivity. The fluorescence intensity of fluorescein dye attached to the chitosan backbone remains constant as an internal reference, while the Eu3+ emission increased linearly upon the consecutive addition of DPA. The selectivity studies were performed by adding different competitive aromatic ligands to the sensing environment and no signifacant fluorescence response was observed. The results demonstrated the superior selectivity of the system to DPA. Overall, this novel chitosan-based ratiometric fluorescent probe enables ratiometric and sensitive DPA detection over nanomolar concentrations (as low as 10 nM) and displays straightforward selectivity over other competitive aromatic ligands.
Açıklama
WOS: 000416369800026
PubMed: 29103500
PubMed: 29103500
Anahtar Kelimeler
Anthrax biomarker, Europium, Chitosan, Fluorescence, Time-resolved
Kaynak
Carbohydrate Polymers
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
180
