Bexarotene inhibits cell proliferation by inducing oxidative stress, DNA damage and apoptosis via PPAR gamma/ NF-kappa B signaling pathway in C6 glioma cells

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dc.authoridKacar, Sedat/0000-0002-0671-8529
dc.authoridkar, fatih/0000-0001-8356-9806
dc.authoridSAHINTURK, VAROL/0000-0003-2317-3644
dc.authoridHacioglu, Ceyhan/0000-0002-0993-6118
dc.authorwosidKacar, Sedat/I-2544-2019
dc.authorwosidkar, fatih/AAI-4540-2021
dc.authorwosidkar, fatih/AAN-4285-2021
dc.authorwosidSAHINTURK, VAROL/V-5195-2017
dc.contributor.authorHacioglu, Ceyhan
dc.contributor.authorKar, Fatih
dc.contributor.authorKacar, Sedat
dc.contributor.authorSahinturk, Varol
dc.contributor.authorKanbak, Gungor
dc.date.accessioned2021-12-01T18:49:32Z
dc.date.available2021-12-01T18:49:32Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractGliomas are one of the most aggressive brain tumors with a poor prognosis in the central nervous system. Bexarotene is a third-generation retinoid X receptor agonist that is promising in the treatment of both cancer and neurodegenerative diseases. In this study, we aimed to investigate the cytotoxic and anti-proliferative effects of bexarotene in C6 glioma cells through the PPAR gamma/NF-kappa B pathway. In the study, first cytotoxic bexarotene concentrations for C6 cells were detected, and then apoptosis profile, reactive oxygen species (ROS), total antioxidant (TAS), 8-hydroxy-2 '-deoxyguanosine (8-OHdG) and nuclear factor-kappa B (NF-kappa B) levels in the cells were determined. In addition, peroxisome proliferator-activated receptor gamma (PPAR gamma) mRNA expression analysis was carried out. As a result, we detected concentration- and time-dependent antiproliferative effects of bexarotene on C6 cells. We found that bexarotene treatment decreased NF-kappa B and TAS levels and increased PPAR gamma and 8-OHdG levels in C6 cells. Bexarotene enhanced PPAR gamma expression in a dose-dependent manner when compared to the control group (P < 0.01). Furthermore, we determined that bexarotene-induced apoptotic C6 cells enhanced through Annexin V-FITC/PI staining and caspase-3/-7 activation analyses since phosphatidylserine level on the outer surface of the cell membrane and caspase-3/-7 activities were increased in the cells treated with bexarotene. In conclusion, bexarotene treatment in C6 glioma cells could modulate apoptosis profile, DNA damage, ROS production, and reduction of TAS levels through inhibition of NF-kappa B by enhancing PPAR gamma expression.en_US
dc.identifier.doi10.1007/s12032-021-01476-z
dc.identifier.issn1357-0560
dc.identifier.issn1559-131X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85101114648en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s12032-021-01476-z
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10739
dc.identifier.volume38en_US
dc.identifier.wosWOS:000619456600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofMedical Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBexaroteneen_US
dc.subjectRXRen_US
dc.subjectGliomaen_US
dc.subjectApoptosisen_US
dc.subjectOxidative stressen_US
dc.subject8-hydroxydeoxyguanosineen_US
dc.subjectPpar&#947en_US
dc.subjectNF-&#954en_US
dc.subjectben_US
dc.titleBexarotene inhibits cell proliferation by inducing oxidative stress, DNA damage and apoptosis via PPAR gamma/ NF-kappa B signaling pathway in C6 glioma cellsen_US
dc.typeArticleen_US

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