Genome-wide exploration of metal tolerance protein (MTP) genes in common wheat (Triticum aestivum): insights into metal homeostasis and biofortification

Basit Öğe Kaydı
dc.contributor.authorVatansever, Recep
dc.contributor.authorFiliz, Ertuğrul
dc.contributor.authorEroğlu, Seçkin
dc.date.accessioned2020-05-01T12:10:28Z
dc.date.available2020-05-01T12:10:28Z
dc.date.issued2017
dc.departmentDÜ, Çilimli Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.descriptionEroglu, Seckin/0000-0002-9494-7080; Filiz, Ertugrul/0000-0001-9636-6389en_US
dc.descriptionWOS: 000398709100007en_US
dc.descriptionPubMed: 28150142en_US
dc.description.abstractMetal transport process in plants is a determinant of quality and quantity of the harvest. Although it is among the most important of staple crops, knowledge about genes that encode for membrane-bound metal transporters is scarce in wheat. Metal tolerance proteins (MTPs) are involved in trace metal homeostasis at the sub-cellular level, usually by providing metal efflux out of the cytosol. Here, by using various bioinformatics approaches, genes that encode for MTPs in the hexaploid wheat genome (Triticum aestivum, abbreviated as Ta) were identified and characterized. Based on the comparison with known rice MTPs, the wheat genome contained 20 MTP sequences; named as TaMTP1-8A, B and D. All TaMTPs contained a cation diffusion facilitator (CDF) family domain and most members harbored a zinc transporter dimerization domain. Based on motif, phylogeny and alignment analysis, A, B and D genomes of TaMTP3-7 sequences demonstrated higher homology compared to TaMTP1, 2 and 8. With reference to their rice orthologs, TaMTP1s and TaMTP8s belonged to Zn-CDFs, TaMTP2s to Fe/Zn-CDFs and TaMTP3-7s to Mn-CDFs. Upstream regions of TaMTP genes included diverse cis-regulatory motifs, indicating regulation by developmental stage, tissue type and stresses. A scan of the coding sequences of 20 TaMTPs against published miRNAs predicted a total of 14 potential miRNAs, mainly targeting the members of most diverged groups. Expression analysis showed that several TaMTPs were temporally and spatially regulated during the developmental time-course. In grains, MTPs were preferentially expressed in the aleurone layer, which is known as a reservoir for high concentrations of iron and zinc. The work identified and characterized metal tolerance proteins in common wheat and revealed a potential involvement of MTPs in providing a sink for trace element storage in wheat grains.en_US
dc.description.sponsorshipScientific and Technological Council of Turkey (Ankara, Turkey) [BIDEB-2232]en_US
dc.description.sponsorshipWe thank Benjamin Gruber for the fruitful discussion and proofreading of the article. Seckin Eroglu thanks Scientific and Technological Council of Turkey (Ankara, Turkey) for the fellowship through BIDEB-2232 program.en_US
dc.identifier.doi10.1007/s10534-017-9997-xen_US
dc.identifier.endpage235en_US
dc.identifier.issn0966-0844
dc.identifier.issn1572-8773
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage217en_US
dc.identifier.urihttps://doi.org/10.1007/s10534-017-9997-x
dc.identifier.urihttps://hdl.handle.net/20.500.12684/6211
dc.identifier.volume30en_US
dc.identifier.wosWOS:000398709100007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiometalsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCDFen_US
dc.subjectMicronutrienten_US
dc.subjectAleuroneen_US
dc.subjectDeficiencyen_US
dc.subjectBiofortificationen_US
dc.titleGenome-wide exploration of metal tolerance protein (MTP) genes in common wheat (Triticum aestivum): insights into metal homeostasis and biofortificationen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
6911.pdf
Boyut:
3.17 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu