Gene expression analysis of MCF7 cell lines of breast cancer treated with herbal extract of Cissampelos pareira revealed association with viral diseases

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dc.contributor.authorGuven, Emine
dc.date.accessioned2021-12-01T18:47:21Z
dc.date.available2021-12-01T18:47:21Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractBackground: It is necessary to assess the cellular, molecular, and pathogenetic characteristics of COVID-19 and attention is required to understand highly effective gene targets and mechanisms. In this study, we suggest understandings into the fundamental pathogenesis of COVID-19 through gene expression analyses using the microarray data set GSE156445 publicly reachable at NIH/NCBI Gene Expression Omnibus database. The data set consists of MCF7 which is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors. The cell lines treated with different quantities of Cissampelos pareira (Cipa). Cipa is a traditional medicinal plant which would possess an antiviral potency in preventing viral diseases such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Methods: Utilizing Biobase, GEOquery, gplots packages in R studio, the differentially expressed genes (DEGs) were identified. The gene ontology (GO) of pathway enrichments employed by utilizing DAVID and KEGG enrichment analyses were studied. We further constructed a human protein-protein interaction (PPI) network and performed, based upon that, a subnetwork module analysis for significant signaling pathways. Results: The study identified 418 differentially expressed genes (DEGs) using bioinformatics tools. The gene ontology of pathway enrichments employed by GO and KEGG enrichment analyses of down-regulated and up-regulated DEGs were studied. Gene expression analysis utilizing gene ontology and KEGG results uncovered biological and signaling pathways such as cell adhesion molecules, plasma membrane adhesion molecules, synapse assembly, and Interleukin-3-mediated signaling which are mostly linked to COVID-19. Our results provide in silico evidence for candidate genes which are vital for the inhibition, adhesion, and encoding cytokine protein including LYN, IGFBP5, IL-1R1, and IL-13RA1 that may have strong biomarker potential for infectious diseases such as COVID-19 related therapy targets.en_US
dc.identifier.doi10.1016/j.genrep.2021.101169
dc.identifier.issn2452-0144
dc.identifier.pmid33907720en_US
dc.identifier.scopus2-s2.0-85114130490en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.1016/j.genrep.2021.101169
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10242
dc.identifier.volume23en_US
dc.identifier.wosWOS:000663413900019en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGuven, Emine
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofGene Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMCF7 cell linesen_US
dc.subjectBiomarkeren_US
dc.subjectIL-1R1en_US
dc.subjectIL-13RA1en_US
dc.subjectViral and infectious diseasesen_US
dc.subjectCOVID-19en_US
dc.subjectAdhesionen_US
dc.subjectCovid-19en_US
dc.subjectRooten_US
dc.titleGene expression analysis of MCF7 cell lines of breast cancer treated with herbal extract of Cissampelos pareira revealed association with viral diseasesen_US
dc.typeArticleen_US

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