Antimicrobial effectiveness of different irrigation activation techniques on teeth with artificial internal root resorption and contaminated with Enterococcus faecalis: a confocal laser scanning,icroscopy analysis

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dc.authorscopusid58153306900
dc.authorscopusid57201546829
dc.authorscopusid56049522100
dc.contributor.authorAkdere, S.K.
dc.contributor.authorAydın, Z.U.
dc.contributor.authorErdönmez, D.
dc.date.accessioned2023-07-26T11:55:22Z
dc.date.available2023-07-26T11:55:22Z
dc.date.issued2023
dc.departmentDÜ, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümüen_US
dc.description.abstractThis study aimed to compare the antibacterial efficacy of standard needle irrigation (SNI), EDDY, passive ultrasonic irrigation (PUI), photon-induced photoacoustic streaming (PIPS), and shock wave enhanced emission photoacoustic streaming (SWEEPS) activation on the teeth with simulated internal root resorption (IRR) and contaminated with Enterococcus faecalis (E. faecalis) using confocal laser scanning microscopy (CLSM) analyses. A total of 79 human maxillary central incisors with a single canal were selected. The canals were accessed, and then, the roots were split in the bucco-lingual direction. Artificial IRR cavities (depth of 0.8 mm and a diameter of 1.6 mm) were prepared using round burs and 20% nitric acid in the middle region of the root halves. The root halves were reconstructed with cyanoacrylate glue, and the canals were contaminated with a culture of E. faecalis for 30 days. Root canal preparation was performed using the ProTaper Next rotary files up to X5 and 2.5% NaOCl irrigation. Teeth were randomly assigned to five groups according to the irrigation activation method (n = 15): SNI, EDDY, PUI, PIPS, and SWEEPS. The final irrigation procedures were performed using a total of 6 mL of 2.5% NaOCl for each tooth with an activation time of 3 × 30 s. The canals were stained with LIVE/DEAD BacLight dye and analyzed with CLSM to determine the percentages of dead bacteria in the biofilm. Two-way ANOVA and post hoc Tukey tests were used for statistical analysis (P <.05). None of the irrigation activation methods tested provided 100% bacterial elimination. There was no significant difference between the irrigation activation methods tested in terms of the percentage of dead bacteria (P > 0.05). In irrigation activation methods other than PIPS, there was no significant difference in the percentage of dead bacteria between the coronal, middle, and apical regions of the roots (P > 0.05). A higher percentage of dead bacteria was found in the middle region compared to the apical region in the PIPS (P < 0.05). Within the limitations of this study, SII, EDDY, PUI, PIPS, and SWEEPS have a similar antimicrobial effect on the teeth with IRR and contaminated with E. faecalis. © 2023, The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.en_US
dc.description.sponsorshipAbant Izzet Baysal Üniversitesi, AIBÜ: 2020.06.02.1460en_US
dc.description.sponsorshipThis study was founded by Bolu Abant Izzet Baysal University, Scientific Research Projects Coordinatorship (Project No: 2020.06.02.1460).en_US
dc.identifier.doi10.1007/s10103-023-03748-8
dc.identifier.issn0268-8921
dc.identifier.issue1en_US
dc.identifier.pmid36943498en_US
dc.identifier.scopus2-s2.0-85150669553en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1007/s10103-023-03748-8
dc.identifier.urihttps://hdl.handle.net/20.500.12684/13058
dc.identifier.volume38en_US
dc.identifier.wosWOS:000954417900001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorErdönmez, D.
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media Deutschland GmbHen_US
dc.relation.ispartofLasers in Medical Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectConfocal laser scanning microscopyen_US
dc.subjectEDDYen_US
dc.subjectEnterococcus faecalisen_US
dc.subjectInternal root resorptionen_US
dc.subjectPIPSen_US
dc.subjectSWEEPSen_US
dc.subjectantiinfective agenten_US
dc.subjectbiomedical and dental materialsen_US
dc.subjecthypochlorite sodiumen_US
dc.subjectdental pulp cavityen_US
dc.subjectEnterococcus faecalisen_US
dc.subjecthumanen_US
dc.subjectlavageen_US
dc.subjectproceduresen_US
dc.subjectroot canal preparationen_US
dc.subjectsolid state laseren_US
dc.subjecttooth diseaseen_US
dc.subjectAnti-Bacterial Agentsen_US
dc.subjectDental Pulp Cavityen_US
dc.subjectEnterococcus faecalisen_US
dc.subjectHumansen_US
dc.subjectLasers, Solid-Stateen_US
dc.subjectRoot Canal Irrigantsen_US
dc.subjectRoot Canal Preparationen_US
dc.subjectRoot Resorptionen_US
dc.subjectSodium Hypochloriteen_US
dc.subjectTherapeutic Irrigationen_US
dc.titleAntimicrobial effectiveness of different irrigation activation techniques on teeth with artificial internal root resorption and contaminated with Enterococcus faecalis: a confocal laser scanning,icroscopy analysisen_US
dc.typeArticleen_US

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