Unveiling Genetic Diversity Among Bacterial Isolates Using SCoT Markers

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dc.authoridAlkan, Mehtap/0000-0002-7923-8892
dc.authoridOZER, Goksel/0000-0002-3385-2520
dc.authoridCANIK OREL, DIDEM/0000-0001-8140-379X
dc.authoridGasic, Katarina/0000-0002-2280-4582
dc.authoridArdic, Mustafa/0000-0002-4193-1990
dc.authoridKovacs, Tamas/0000-0001-6647-8136
dc.contributor.authorTekin, Fatih
dc.contributor.authorAltin, Irem
dc.contributor.authorAydin, Furkan
dc.contributor.authorAlkan, Mehtap
dc.contributor.authorOrel, Didem Canik
dc.contributor.authorArdic, Mustafa
dc.contributor.authorGasic, Katarina
dc.date.accessioned2025-10-11T20:48:47Z
dc.date.available2025-10-11T20:48:47Z
dc.date.issued2024
dc.departmentDüzce Üniversitesien_US
dc.description.abstractThis study aimed to unveil the genetic diversity among 47 bacterial isolates from various species using start codon targeted (SCoT) markers. Six SCoT primers yielded 219 reproducible bands, with 89.04% exhibiting polymorphism. The amplification process generated 28 to 50 fragments per primer, with an average of 36.50. Genetic diversity was quantified using polymorphic information content (PIC) values ranging from 0.11 to 0.14, with SCoT32 showing the highest PIC (0.14) and SCoT23 the lowest (0.11). The resolving power (RP) index, used to assess primer discriminatory power, varied significantly, with SCoT23 demonstrating the highest RP (6.00) and SCoT29 the lowest (4.51). Comparative analysis with conventional markers like M13 and (GTG)5 revealed that certain SCoT primers exhibited superior PIC values, which indicates enhanced utility for interspecies differentiation. The high discrimination level achieved by SCoT primers underscores their effectiveness in genetic differentiation and biodiversity assessment within bacterial populations. This research highlights SCoT markers as powerful tools for microbial genetic studies, which offers valuable insights into bacterial diversity and provides a robust methodological framework for future investigations aimed at elucidating genetic variation and improving species identification. The application of SCoT markers represents a significant advancement in molecular techniques for bacterial characterization and phylogenetic analysis, demonstrating their potential to enhance our understanding of microbial genetics and evolution.en_US
dc.description.sponsorshipReggio Emilia, Italyen_US
dc.description.sponsorshipAuthors are highly thankful to Prof Dr Emilio Stefani, Departments of Life Sciences, University of Modena and Reggio Emilia, Italy for providing X. arboricola pv. juglandis isolates.en_US
dc.identifier.doi10.1007/s00284-024-03938-9
dc.identifier.issn0343-8651
dc.identifier.issn1432-0991
dc.identifier.issue12en_US
dc.identifier.pmid39407052en_US
dc.identifier.scopus2-s2.0-85206449543en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1007/s00284-024-03938-9
dc.identifier.urihttps://hdl.handle.net/20.500.12684/22102
dc.identifier.volume81en_US
dc.identifier.wosWOS:001333495900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCurrent Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250911
dc.titleUnveiling Genetic Diversity Among Bacterial Isolates Using SCoT Markersen_US
dc.typeArticleen_US

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