Erdosteine reduces cytotoxicity induced by alpha- and beta-amanitin, but not gamma-amanitin, in CA3 hepatocyte cultures

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dc.authoridkaya, ertugrul/0000-0003-0081-682X
dc.authoridYılmaz, İsmail/0000-0002-4474-9617
dc.authorwosidkaya, ertugrul/C-1505-2013
dc.contributor.authorBayram, Recep
dc.contributor.authorYılmaz, İsmail
dc.contributor.authorYaykasli, Kursat Oguz
dc.contributor.authorKaya, Ertuğrul
dc.date.accessioned2023-07-26T11:54:28Z
dc.date.available2023-07-26T11:54:28Z
dc.date.issued2022
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalıen_US
dc.description.abstractAmanitin poisoning still has no particular, effective antidote. Erdosteine has been shown to protect numerous tissues, particularly those in the liver. This study investigates the potential therapeutic effects of erdosteine on alpha-, beta-and gamma-amanitin-induced hepatotoxicity in in vitro models. Three hours after administering amatoxins at various concentrations (1-50 mu g/mL) to the cells of the C3A human hepatocyte cell line, erdosteine was administered in different concentrations (i.e., 1, 10, 50, 100 and 250 mu g/mL). The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was selected to determine cell viability. When concentrations of 1, 10, 50, 100 and 250 mu g/mL of erdosteine were applied to cell lines, the following cell viability rates were obtained: 106%,99%,93%,86% and 86%, respectively, at a 10 mu g/mL alpha-amanitin-induced toxicity; 43%,41%,41%,37% and 35%, respectively, at a 25 mu g/mL alpha-amanitin-induced toxicity; 44%,42%,41%,39% and 41%, respectively, at a 50 mu g/mL alpha-amanitin-induced toxicity; 136%,142%,143%,137% and 120%, respectively, at a 10 mu g/mL beta-amanitin-induced toxicity; 113%,107%,107%,106% and 86%, respectively, at a 25 mu g/mL beta-amanitin-induced toxicity; 78%,77%,77%,74% and 70%, respectively, at a 10 mu g/mL gammaamanitin-induced toxicity; and 39%,40%,39%,35% and 31%, respectively, at a 25 mu g/mL gamma-amanitininduced toxicity. This study was the first to evaluate the in vitro efficacy of erdosteine in cytotoxicity induced by alpha-, beta-and gamma-amanitin. Non-high (low and medium) doses of erdosteine are capable of nearly entirely preventing toxicity at mild hepatotoxic concentrations caused by amatoxin and partially preventing toxicity at moderate and severe concentrations. The beneficial effects of erdosteine, especially on the toxicity of alpha-and beta-amanitin, are promising.en_US
dc.identifier.doi10.1016/j.toxicon.2022.04.009
dc.identifier.endpage58en_US
dc.identifier.issn0041-0101
dc.identifier.issn1879-3150
dc.identifier.pmid35443191en_US
dc.identifier.scopus2-s2.0-85128850813en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage52en_US
dc.identifier.urihttps://doi.org/10.1016/j.toxicon.2022.04.009
dc.identifier.urihttps://hdl.handle.net/20.500.12684/12842
dc.identifier.volume213en_US
dc.identifier.wosWOS:000793768800007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYaykaşlı, Kürşat Oğuz
dc.institutionauthorKaya, Ertuğrul
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofToxiconen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectAlpha-Amanitin; Beta-Amanitin; Gamma-Amanitin; Mtt Assay; C3a Human; Hepatocyte Cell Line; Erdosteineen_US
dc.subjectInduced Hepatotoxicity; Oxidative Stress; N-Acetylcysteine; Oxidant Injury; Purification; Phalloides; Cimetidine; Toxicity; Liver; Aciden_US
dc.titleErdosteine reduces cytotoxicity induced by alpha- and beta-amanitin, but not gamma-amanitin, in CA3 hepatocyte culturesen_US
dc.typeArticleen_US

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