Determination of the dose-dependent toxic effects of mad honey on mouse liver using ATR-FTIR spectroscopy

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dc.contributor.authorCakmak-Arslan, Gulgun
dc.contributor.authorHaksoy, Humeyra
dc.contributor.authorGoc-Rasgele, Pinar
dc.contributor.authorKekecoglu, Meral
dc.date.accessioned2021-12-01T18:47:24Z
dc.date.available2021-12-01T18:47:24Z
dc.date.issued2020
dc.department[Belirlenecek]en_US
dc.description.abstractMad honey (MH) is obtained from Rhododendron plants, which are extensively grown in some regions of the world such as Europe, North America, Tropical Asia and Turkey. Although it has been known that MH induces adverse effects in the body due to grayanotoxin (GTX) in it, it is widely used for some medical purposes by the public. In this study, the effects of MH (25, 50 and 75 mg/kg) and GTX-III (0.01 mg/kg), which is the pure form of the most toxic type of the GTXs in MH, were investigated on the mouse liver at molecular level via Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) spectroscopy. The results showed that 25 and 50 mg/kg of MH didn't cause any significant alterations in the liver tissue except a decrease in the glycogen amount. However, significant differences were observed between 75 mg/kg MH and GTX-III treated groups and control group. For example, the amounts of saturated lipids, nucleic acids and proteins increased in the 75 mg/kg MH and GTX-III treated groups. A decrease in the ratios of unsaturated/saturated lipid, CH2/lipid and carbonyl/lipid and an increase in the ratio of CH3/lipid were observed after the administration of 75 mg/kg MH and GTX-III, all of which may be a consequence of lipid peroxidation. Moreover, 75 mg/kg MH and GTX-III caused a decrease in the membrane order, an increase in the membrane fluidity and some important changes on the secondary structure of proteins indicating protein denaturation. In addition, Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA) confirmed these findings. These results revealed that MH induces significant dose-dependent toxic effects in the structure and function of the liver tissue. This study also showed that ATR-FTIR spectroscopy provides a rapid and sensitive monitoring of the changes induced by a toxic compound on biological tissues at molecular level. (c) 2019 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.saa.2019.117719
dc.identifier.issn1386-1425
dc.identifier.issn1873-3557
dc.identifier.pmid31753646en_US
dc.identifier.scopus2-s2.0-85075420065en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.saa.2019.117719
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10256
dc.identifier.volume228en_US
dc.identifier.wosWOS:000513010500040en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofSpectrochimica Acta Part A-Molecular And Biomolecular Spectroscopyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMad honeyen_US
dc.subjectGrayanotoxin (GTX)en_US
dc.subjectATR-FTIR spectroscopyen_US
dc.subjectLiveren_US
dc.subjectHierarchical cluster analysis (HCA)en_US
dc.subjectPrincipal component analysis (PCA)en_US
dc.subjectRainbow-Trout Liveren_US
dc.subjectRhododendronen_US
dc.subjectIntoxicationen_US
dc.subjectBlooden_US
dc.subjectMembranesen_US
dc.subjectDamageen_US
dc.subjectGrayanotoxinsen_US
dc.subjectParametersen_US
dc.subjectExpressionen_US
dc.subjectSteatosisen_US
dc.titleDetermination of the dose-dependent toxic effects of mad honey on mouse liver using ATR-FTIR spectroscopyen_US
dc.typeArticleen_US

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