Boric Acid Suppresses Cell Survival by Triggering Endoplasmic Reticulum Stress-Induced Autophagy in Cervical Cancers

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dc.contributor.authorKeyif, Betul
dc.contributor.authorHacioglu, Ceyhan
dc.date.accessioned2025-10-11T20:48:13Z
dc.date.available2025-10-11T20:48:13Z
dc.date.issued2025
dc.departmentDüzce Üniversitesien_US
dc.description.abstractCervical cancer ranks as the fourth most common cancer amongst women globally. This study aimed to investigate boric acid's effects on endoplasmic reticulum (ER) stress and autophagy signalling pathways in cervical cancer cells. We first assessed boric acid's effects on cell viability and proliferation in HUF and HeLa cell lines. Subsequently, we analysed cyclin D1 and CDK4 levels and boric acid-induced nuclear morphology changes. We then examined autophagosome formation and mRNA expression of autophagy/ER stress markers (Beclin1, p62, LC3-I/II, GRP78, p-IRE1 alpha, p-PERK, CHOP and cleaved-caspase-3) in HeLa cells. The findings revealed that boric acid's IC50 was 3.17 mM for HUF cells but significantly lower (641.2 mu M) for HeLa cells, indicating cancer cell sensitivity. In HeLa cells, boric acid-induced a dose-dependent decrease in cyclin D1 and CDK4 levels (associated with G1 phase arrest), which we did not observe in HUF cells. Additionally, boric acid treatment caused nuclear abnormalities in HeLa cells. Boric acid promoted autophagy by enhancing autophagosome formation and upregulating Beclin1, p62, and LC3-I/II expression. Concurrently, it induced ER stress by increasing GRP78, p-IRE1 alpha, p-PERK and CHOP expression. Furthermore, boric acid increased cleaved-caspase-3 expression and apoptotic cell counts. In conclusion, this study underscores boric acid's potential therapeutic effects in cervical cancer through ER stress and autophagy regulation.en_US
dc.identifier.doi10.1111/jcmm.70740
dc.identifier.issn1582-1838
dc.identifier.issn1582-4934
dc.identifier.issue14en_US
dc.identifier.pmid40682482en_US
dc.identifier.scopus2-s2.0-105011271403en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1111/jcmm.70740
dc.identifier.urihttps://hdl.handle.net/20.500.12684/21810
dc.identifier.volume29en_US
dc.identifier.wosWOS:001531151600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Cellularand Molecular Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250911
dc.subjectautophagyen_US
dc.subjectboric aciden_US
dc.subjectcervical canceren_US
dc.subjectendoplasmic reticulum stressen_US
dc.titleBoric Acid Suppresses Cell Survival by Triggering Endoplasmic Reticulum Stress-Induced Autophagy in Cervical Cancersen_US
dc.typeArticleen_US

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