GpEF1A: a novel lysine methyltransferase gene from Gypsophila perfoliata L. involved in boron homeostasis

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dc.authoridFiliz, Ertugrul/0000-0001-9636-6389en_US
dc.authoridDogu, Suleyman/0000-0002-5352-9288en_US
dc.authoridBOR, MELIKE/0000-0002-0170-2800en_US
dc.authorscopusid8856341800en_US
dc.authorscopusid59139344200en_US
dc.authorscopusid59139518900en_US
dc.authorscopusid23984566200en_US
dc.authorscopusid34881443900en_US
dc.authorscopusid7003854062en_US
dc.authorwosidFiliz, Ertugrul/I-9440-2016en_US
dc.contributor.authorAkbudak, M. A.
dc.contributor.authorCirik, N.
dc.contributor.authorErdeger, S. N.
dc.contributor.authorFiliz, E.
dc.contributor.authorDogu, S.
dc.contributor.authorBor, M.
dc.date.accessioned2024-08-23T16:04:15Z
dc.date.available2024-08-23T16:04:15Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractRapid accumulation of boron (B) leads to toxicity in plant tissues, and the narrow gap between deficiency and toxicity makes it difficult to adjust essential B levels in soil for plant productivity. Therefore, understanding different aspects of B tolerance is necessary to provide new and valid solutions to B toxicity. Gypsophila perfoliata stands out as a remarkable example of a B-tolerant plant, with a natural propensity to thrive in environments such as B mines and soils enriched with high levels of B. In this study, a yeast functional screening experiment was conducted using cDNA libraries from G. perfoliata leaf and root cells for B tolerance. Ten colonies from the leaf library grew in 80 mm boric acid, while none emerged from the root library. Analysis of isolated cDNAs showed identical sequences and a unique motif related to B tolerance. The gene GpEF1A was identified in the tolerant yeast colonies, with predicted structural features suggesting its role, and RT-qPCR indicating increased expression under B stress. A regulatory role for EF1A lysine methylation was proposed in mammalian cells and fungi because of its dynamic and inducible nature under environmental constraints. This could also be relevant for plant cells, as the high similarity of the GpEF1A gene in some salt-tolerant plants might indicate the upregulation of EF1A as a conserved way to cope with abiotic stress conditions. This report represents the first instance of involvement of GpEF1A in B tolerance, and further detailed studies are necessary to understand other components of this tolerance mechanism.en_US
dc.description.sponsorshipAkdeniz University Scientific Research Projects Coordination Unit [FYL-2022-6079]; Scientific and Technological Research Council of Turkiye (TUBITAK)en_US
dc.description.sponsorshipThis study was supported by Akdeniz University Scientific Research Projects Coordination Unit grant no. FYL-2022-6079. The work was carried out in the framework of COST Action 19116 - Trace Metal Metabolism in Plants (PLANTMETALS). Open access funding provided by the Scientific and Technological Research Council of Turkiye (TUBITAK).en_US
dc.identifier.doi10.1111/plb.13658
dc.identifier.endpage734en_US
dc.identifier.issn1435-8603
dc.identifier.issn1438-8677
dc.identifier.issue5en_US
dc.identifier.pmid38781082en_US
dc.identifier.scopus2-s2.0-85193958869en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage727en_US
dc.identifier.urihttps://doi.org/10.1111/plb.13658
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14124
dc.identifier.volume26en_US
dc.identifier.wosWOS:001229763600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofPlant Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAbiotic stressen_US
dc.subjectEF-Tuen_US
dc.subjectlysine methylationen_US
dc.subjectpost-translational modificationen_US
dc.subjectSynthesis Elongation-Factoren_US
dc.subjectFactor Ef-Tuen_US
dc.subjectHeat-Stressen_US
dc.subjectExpressionen_US
dc.subjectBarleyen_US
dc.subjectWheaten_US
dc.subjectTransporteren_US
dc.subjectToleranceen_US
dc.subjectMaizeen_US
dc.subjectMethylationen_US
dc.titleGpEF1A: a novel lysine methyltransferase gene from Gypsophila perfoliata L. involved in boron homeostasisen_US
dc.typeArticleen_US

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