In Vivo Genotoxicity Testing of Bentazone Herbicide in Danio rerio Erythrocytes Using the Micronucleus and Nuclear Abnormality Assays
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Int Publ Ag
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Bentazone herbicide is in the benzothiadiazole group and is often used for the elimination of weeds. In this experiment, the in vivo genotoxicity of bentazone herbicide in Danio rerio erythrocytes was investigated using micronucleus and nuclear abnormality assays. Fish were exposed to three different concentrations (2.5, 5, and 10 mg/L) for 24, 48, 72, and 96 h, including the control group. When compared with the negative group, it was found that there was a statistical increase in the number of micronuclei in a concentration-dependent manner. According to the data obtained from the erythrocyte nucleolar abnormality assay, the most common abnormality for the 24-h treatment was kidney-shaped nucleus (at 10 mg/L concentration); the most common abnormality for the 48-, 72-, and 96-h treatment periods was echinocyte (at 5 and 10 mg/L concentrations). All concentrations of bentazone herbicide caused an increase in the total abnormality level in Danio rerio erythrocytes at all treatment times. These increases were concentration dependent for 24-, 72-, and 96-h treatment times, except for the 48-h treatment. Furthermore, for all treatment groups, the high concentration of bentazone herbicide (10 mg/L) caused changes on the long-short axis of the erythrocytes and the long-short axis of the nucleus, but these changes were not statistically significant. The data we obtained as a result of the study showed that bentazone herbicide is genotoxic for aquatic organisms depending on their use. Further research into different aquatic organisms is needed to investigate the genotoxicity mechanisms of bentazone herbicide in aquatic organisms.
Açıklama
Anahtar Kelimeler
Herbicide, Erythrocyte, Genotoxicity, Danio rerio, Micronucleus assay, Pesticides
Kaynak
Water Air and Soil Pollution
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
235
Sayı
1
