Evaluation of possible cytotoxic, genotoxic and epigenotoxic effects of titanium dioxide nanoparticles and possible protective effect of melatonin

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dc.authoridOral, Didem/0000-0002-7025-6576en_US
dc.authoridbalci ozyurt, aylin/0000-0002-0060-271Xen_US
dc.authoridZeybek, Dilara/0000-0002-6161-5661en_US
dc.authoridCAKIR, Deniz Arca/0000-0002-6110-1775en_US
dc.authorscopusid58062075500en_US
dc.authorscopusid57211079309en_US
dc.authorscopusid57482646800en_US
dc.authorscopusid8540689700en_US
dc.authorscopusid57188932723en_US
dc.authorscopusid35105891500en_US
dc.authorscopusid35239631400en_US
dc.authorwosidOral, Didem/KFS-7208-2024en_US
dc.authorwosidbalci ozyurt, aylin/HKE-3259-2023en_US
dc.contributor.authorBalci-Ozyurt, Aylin
dc.contributor.authorYirun, Anil
dc.contributor.authorCakir, Deniz Arca
dc.contributor.authorZeybek, N. Dilara
dc.contributor.authorOral, Didem
dc.contributor.authorSabuncuoglu, Suna
dc.contributor.authorErkekoglu, Pinar
dc.date.accessioned2024-08-23T16:04:27Z
dc.date.available2024-08-23T16:04:27Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractNanoparticles (NPs) are particles of matter that are between 1 to 100 nm in diameter. They are suggested to cause toxic effects in both humans and environment thorough different mechanisms. However, their toxicity profile may be different from the parent material. Titanium dioxide (TiO2) NPs are widely used in cosmetic, pharmaceutical and food industries. As a white pigment, the use of TiO2 is used in food coloring, industrial paints, clothing and UV filters has increased tremendously in recent years. Melatonin, on the other hand, is a well-known antioxidant and may prevent oxidative stress caused by a variety of different substances, including NPs. In the current study, we aimed to comparatively investigate the effects of normal-sized TiO2 (220 nm) and nano-sized TiO2 (21 nm) on cytopathology, cytotoxicity, oxidative damage (lipid peroxidation, protein oxidation and glutathione), genotoxicity (8-hydroxydeoxyguanosine), apoptosis (caspase 3, 8 and 9) and epigenetic alterations (global DNA methylation, H3 acetylation) on 3T3 fibroblast cells. In addition, the possible protective effects of melatonin, which is known to have strong antioxidant effects, against the toxicity of TiO2 were also evaluated. Study groups were: a. the control group; b. melatonin group; c. TiO2 group; d. nano-sized TiO2 group; e. TiO2 + melatonin group and f. nano-sized TiO2 + melatonin group. We observed that both normal-sized and nano-sized TiO2 NPs showed significant toxic effects. However, TiO2 NPs caused higher DNA damage and global DNA methylation compared to normal-sized TiO2 whereas normal-sized TiO2 led to lower H3 acetylation vs. TiO2 NPs. Melatonin showed partial protective effect against the toxicity caused by TiO2 NPs.en_US
dc.description.sponsorshipHacettepe University Scientific Research Unit Research Project [THD-2019-18423]en_US
dc.description.sponsorshipThis study is supported by Hacettepe University Scientific Research Unit Research Project. Project No: THD-2019-18423.en_US
dc.identifier.doi10.1080/15376516.2023.2259980
dc.identifier.endpage121en_US
dc.identifier.issn1537-6516
dc.identifier.issn1537-6524
dc.identifier.issue2en_US
dc.identifier.pmid37794599en_US
dc.identifier.scopus2-s2.0-85173538882en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage109en_US
dc.identifier.urihttps://doi.org/10.1080/15376516.2023.2259980
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14196
dc.identifier.volume34en_US
dc.identifier.wosWOS:001078291000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofToxicology Mechanisms And Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTitanium dioxideen_US
dc.subjectnanoparticleen_US
dc.subjectoxidative damageen_US
dc.subjectepigenetic alterationsen_US
dc.subjectapoptosisen_US
dc.subjectInduce Dna-Damageen_US
dc.subjectIn-Vitroen_US
dc.subjectHealthy-Individualsen_US
dc.subjectTio2 Nanoparticlesen_US
dc.subjectOxidative Stressen_US
dc.subjectCellsen_US
dc.subjectInflammationen_US
dc.subjectMethylationen_US
dc.subjectAntioxidanten_US
dc.subjectToxicityen_US
dc.titleEvaluation of possible cytotoxic, genotoxic and epigenotoxic effects of titanium dioxide nanoparticles and possible protective effect of melatoninen_US
dc.typeArticleen_US

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