Evaluation of Genotoxic and Cytotoxic Effects of Natamycin in Mice Bone Marrow Cells
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Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Zoological Soc Pakistan
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Natamycin, food preservative, is widely used in food industry against yeast and fungi. The potential genotoxicity of a commercial formulation of natamycin (Delvocid, containing 50% natamycin as the active ingredient) in mice bone marrow cells Was investigated in vivo by chromosome aberrations (CA) and micronucleus assays (MA). Animals were intraperitoneally treated with 200, 400 and 800 mg/kg of natamycin for 6, 12 and 24 h in CA assay, for 24, 48 and 72 hours in MN assay. In the present study, natamycin did not increase chromosome aberrations. It significantly induced frequency of micronucleus at all concentrations for both 24 and 48 h in female mice, at the two highest (400 and 800 mg/kg) concentrations for both 24 and 48 h in male mice when compared with negative control. Mice bone marrow erythrocytes exposed to all concentrations of natamycin showed significant. decreases in mitotic index for all treatment periods. In addition, natamycin reduced polychromatic erytrocyte/normochromatic erytrocyte ratio at all concentrations for 48h and at the highest (800 mg/kg) concentration for 24 and 72h in female mice; at all concentrations for 24 and 48h in male mice. The results of the present study show that a commercial formulation of natamycin was aneugenic and cytotoxic to mice bone marrow in vivo. For this reason it is necessary to be careful when using these chemicals in food as preservatives.
Description
WOS: 000328301100030
Keywords
Natamycin, food additive, chromosome aberration, micronucleus, mice bone marrow
Journal or Series
Pakistan Journal Of Zoology
WoS Q Value
Q4
Scopus Q Value
Q4
Volume
45
Issue
4
