The Characterization and Beta-Lactam Resistance of Staphylococcal Community Recovered from Raw Bovine Milk

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dc.authoridkaya, ertugrul/0000-0003-0081-682Xen_US
dc.authoridSipahi, Nisa/0000-0001-8915-3545en_US
dc.authoridÇELİK DOĞAN, Cansu/0000-0002-9508-7473en_US
dc.authoridPinar, Orhan/0000-0002-1361-8614en_US
dc.authorscopusid57211212241en_US
dc.authorscopusid24724487100en_US
dc.authorscopusid57211514386en_US
dc.authorscopusid55864797400en_US
dc.authorwosidkaya, ertugrul/C-1505-2013en_US
dc.authorwosidSipahi, Nisa/AAG-4583-2019en_US
dc.authorwosidÇELİK DOĞAN, Cansu/AAI-2252-2019en_US
dc.contributor.authorSipahi, Nisa
dc.contributor.authorKaya, Ertugrul
dc.contributor.authorCelik, Cansu
dc.contributor.authorPinar, Orhan
dc.date.accessioned2024-08-23T16:03:37Z
dc.date.available2024-08-23T16:03:37Z
dc.date.issued2023en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractStaphylococci is an opportunistic bacterial population that is permanent in the normal flora of milk and poses a serious threat to animal and human health with some virulence factors and antibiotic-resistance genes. This study was aimed at identifying staphylococcal species isolated from raw milk and to determine hemolysis, biofilm, coagulase activities, and beta-lactam resistance. The raw milk samples were collected from the Duzce (Turkiye) region, and the study data represent a first for this region. The characterization of the bacteria was performed with MALDI-TOF MS and 16S rRNA sequence analysis. The presence of coa, icaB, blaZ, and mecA was investigated with PCR. A nitrocefin chromogenic assay was used for beta-lactamase screening. In this context, 84 staphylococci were isolated from 10 different species, and the dominant species was determined as S. aureus (32.14%). Although 32.14% of all staphylococci were positive for beta hemolysis, the icaB gene was found in 57.14%, coa in 46.42%, mecA in 15.47%, and blaZ in 8.33%. As a result, Staphylococcus spp. strains that were isolated from raw milk in this study contained some virulence factors at a high level, but also contained a relatively low level of beta-lactam resistance genes. However, considering the animal-environment-human interaction, it is considered that the current situation must be monitored constantly in terms of resistance concerns. It must not be forgotten that the development of resistance is in constant change among bacteria.en_US
dc.identifier.doi10.3390/antibiotics12030556
dc.identifier.issn2079-6382
dc.identifier.issue3en_US
dc.identifier.pmid36978423en_US
dc.identifier.scopus2-s2.0-85151333186en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/antibiotics12030556
dc.identifier.urihttps://hdl.handle.net/20.500.12684/13831
dc.identifier.volume12en_US
dc.identifier.wosWOS:000953014200001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofAntibiotics-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectstaphylococcien_US
dc.subjectbiofilmen_US
dc.subjectbeta-lactamaseen_US
dc.subjectcoagulaseen_US
dc.subjectvirulenceen_US
dc.subjectmecAen_US
dc.subjectblaZen_US
dc.subjectAntibiotic-Resistanceen_US
dc.subjectAntimicrobial Susceptibilityen_US
dc.subjectMethicillin-Resistanten_US
dc.subjectBiofilm Formationen_US
dc.subjectEscherichia-Colien_US
dc.subjectAureusen_US
dc.subjectGeneen_US
dc.subjectCowsen_US
dc.subjectIdentificationen_US
dc.subjectRegionen_US
dc.titleThe Characterization and Beta-Lactam Resistance of Staphylococcal Community Recovered from Raw Bovine Milken_US
dc.typeArticleen_US

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