The effects of hydroxytyrosol on Prdx6 and insulin expression in diabetic rat pancreases

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dc.authoridSoylu, Hakan/0000-0002-1177-2351en_US
dc.authorscopusid55982152000en_US
dc.authorscopusid56239473400en_US
dc.authorwosidSoylu, Hakan/I-8911-2017en_US
dc.contributor.authorSoylu, Hakan
dc.contributor.authorKaracor, Kayihan
dc.date.accessioned2024-08-23T16:07:16Z
dc.date.available2024-08-23T16:07:16Z
dc.date.issued2023en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractDiabetes mellitus is a widespread endocrine disease worldwide, accompanying chronic hyperglycemia. In this study, we investigated the effect of hydroxytyrosol, which exerts an antioxidant effect, on the expressions of insulin and peroxiredoxin-6 (Prdx6), which protect cells against oxidative injury in diabetic rat pancreas. This experimental study had four groups with ten animals in each group: control (nondiabetic) group, hydroxytyrosol group [10 mg/kg/day intraperitoneal injection (ip) hydroxytyrosol for 30 days], streptozotocin group (single ip injection of 55 mg/kg streptozotocin), and streptozotocin + hydroxytyrosol group (single ip injection of streptozotocin and ip injection of 10 mg/kg/day hydroxytyrosol for 30 days). During the experiment, blood glucose levels were measured at regular intervals. Insulin expression was determined by immunohistochemistry and Prdx6 expression was determined by immunohistochemistry and western blot. Immunohistochemistry and western blot results were analyzed by one-way ANOVA with applied Holm-Sidak multiple comparison test, and blood glucose results were analyzed by two-way repeated measures ANOVA with applied Tukey's multiple comparison test. Blood glucose levels on days 21 and 28 were significantly lower in the streptozotocin + hydroxytyrosol group compared with the streptozotocin group (day 21, p = 0.049 and day 28, p = 0.003). Expression of both insulin and Prdx6 were lower in the streptozotocin and the streptozotocin + hydroxytyrosol groups compared with the control and hydroxytyrosol groups (p < 0.001). Insulin and Prdx6 expression in the streptozotocin + hydroxytyrosol group were higher compared with the streptozotocin group (p < 0.001). The immunohistochemical findings of Prdx6 and western blot were the same. In conclusion, hydroxytyrosol, which is an antioxidant compound, increased Prdx6 and insulin expression in diabetic rats. Insulin increased by hydroxytyrosol may have been effective in reducing blood glucose levels. Furthermore, hydroxytyrosol may exert its effect on insulin by increasing Prdx6 expression. Thus, hydroxytyrosol may decrease or prevent several hyperglycemia-dependent complications by increasing the expression of these proteins.en_US
dc.identifier.doi10.1007/s00418-023-02207-3
dc.identifier.endpage134en_US
dc.identifier.issn0948-6143
dc.identifier.issn1432-119X
dc.identifier.issue2en_US
dc.identifier.pmid37219732en_US
dc.identifier.scopus2-s2.0-85160253283en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage127en_US
dc.identifier.urihttps://doi.org/10.1007/s00418-023-02207-3
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14546
dc.identifier.volume160en_US
dc.identifier.wosWOS:000992047600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofHistochemistry and Cell Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPrdx6en_US
dc.subjectDiabetesen_US
dc.subjectHydroxytyrosolen_US
dc.subjectStreptozotocinen_US
dc.subjectPancreasen_US
dc.subjectBlood-Glucoseen_US
dc.subjectStressen_US
dc.subjectCytokinesen_US
dc.titleThe effects of hydroxytyrosol on Prdx6 and insulin expression in diabetic rat pancreasesen_US
dc.typeArticleen_US

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