Toxicity, mutagenicity and stability assessment of simply produced electrolyzed water as a wound healing agent in vitro

Basit Öğe Kaydı
dc.authoridReis, Rengin/0000-0002-3484-2201
dc.authoridDinc, Ozge/0000-0002-3029-7840
dc.authoridSipahi, Hande/0000-0001-6482-3143
dc.authoridAydin, Ahmet/0000-0003-3499-6435
dc.authorwosidReis, Rengin/AAI-4743-2020
dc.authorwosidDinc, Ozge/AAB-3602-2020
dc.contributor.authorReis, R.
dc.contributor.authorSipahi, H.
dc.contributor.authorDinc, O.
dc.contributor.authorKavaz, T.
dc.contributor.authorCharehsaz, M.
dc.contributor.authorDimoglo, A.
dc.contributor.authorAydin, A.
dc.date.accessioned2021-12-01T18:49:17Z
dc.date.available2021-12-01T18:49:17Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractOver the last decade, electrolyzed water (EW) produced by salt and tap water has gained importance due to its antimicrobial effects. Regarding to chlorine-based compounds, EW also used in post-harvest safety of food processing and sterilization of surfaces. The latest studies suggested that EW might act as wound healing agent due to anti-infective and cell proliferative properties. In this study, we evaluated acute contact cytotoxicity in L929 mice fibroblast cells and wound healing activity of EWsin vitro.In addition, mutagenic activity was evaluated by Ames test with and without metabolic activation by S9 fraction and the stability profile of freshly prepared EWs has been followed up. According to the results, strong acid (StAEW) and mixed EW (MEW) showed dose-dependent cytotoxicity due to possible high HOCl concentration, while slightly acidic and catholyte EW (CEW) were not cytotoxic even applied directly for 30 sec. Further, StAEW and CEW showed a significant increase in L929 cell migration in scratch assay. Likewise, with/ without metabolic activation, neither of EWs had shown mutagenic profile in TA 98 and TA100 strains ofSalmonella typhimurium.Follow-up of ORP (oxidation-reduction potential), pH and FCC (free chlorine concentration) showed that temperature and light were important storage conditions to maintain a stable profile particularly for ORP and FCC, which are the most important indicators for biological activity of EW. According to the present findings, it can be suggested that particularly StAEW, may represent a valuable wound healing agent with an achievable, economical and easy production system when stored under proper conditions.en_US
dc.description.sponsorshipTUBITAK (Turkish Research and Scientific Council)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [116Z169]en_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This project was financially supported by TUBITAK (Turkish Research and Scientific Council) 1002 program with a Grant No. 116Z169. The authors also thank to Gulenay Alyurt for her considerate effort on EW project.en_US
dc.identifier.doi10.1177/0960327120952151
dc.identifier.endpage463en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue3en_US
dc.identifier.pmid32909829en_US
dc.identifier.scopus2-s2.0-85090558478en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage452en_US
dc.identifier.urihttps://doi.org/10.1177/0960327120952151
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10697
dc.identifier.volume40en_US
dc.identifier.wosWOS:000568535700001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectrolyzed wateren_US
dc.subjectcytotoxicityen_US
dc.subjectL929en_US
dc.subjectAmes testen_US
dc.subjectscratch assayen_US
dc.subjectstabilityen_US
dc.subjectDisinfection By-Productsen_US
dc.subjectOxidizing Wateren_US
dc.subjectPhysical-Propertiesen_US
dc.subjectAqueous-Solutionen_US
dc.subjectAciden_US
dc.subjectMechanismen_US
dc.subjectEfficacyen_US
dc.subjectSafetyen_US
dc.titleToxicity, mutagenicity and stability assessment of simply produced electrolyzed water as a wound healing agent in vitroen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
10697.pdf
Boyut:
938.43 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu