A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder

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dc.authorscopusid55270667200en_US
dc.authorscopusid56682083300en_US
dc.authorscopusid57203489662en_US
dc.authorscopusid57194533999en_US
dc.authorscopusid57209565300en_US
dc.authorscopusid57480732600en_US
dc.contributor.authorOzkan-Kotiloglu, Selin
dc.contributor.authorKaya-Akyuzlu, Dilek
dc.contributor.authorGuven, Emine
dc.contributor.authorDogan, Ozlem
dc.contributor.authorAgtas-Ertan, Ece
dc.contributor.authorOzgur-Ilhan, Inci
dc.date.accessioned2024-08-23T16:07:06Z
dc.date.available2024-08-23T16:07:06Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractBackground Alcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to determine the methylation levels of two crucial genes (GHRL and GHSR) in ghrelin signaling and further investigate the association between methylation ratios and plasma ghrelin levels. Methods Individuals diagnosed with (n = 71) and without (n = 82) AUD were recruited in this study. DNA methylation levels were measured through methylation-sensitive high-resolution melting (MS-HRM). Acylated ghrelin levels were detected by ELISA. The GHRL rs696217 polymorphism was analyzed by the standard PCR-RFLP method. Results GHRL was significantly hypermethylated (P < 0.0022) in AUD between 25 and 50% methylation than in control subjects but no significant changes of GHSR methylation were observed. Moreover, GHRL showed significant positive correlation of methylation ratio between 25 and 50% with age. A significant positive correlation between GHSR methylation and ghrelin levels in the AUD group was determined (P = 0.037). The level of GHRL methylation and the ghrelin levels showed a significant association in the control subjects (P = 0.042). Conclusion GHSR and GHRL methylation levels did not change significantly between control and AUD groups. However, GHRL and GHSR methylations seemed to have associations with plasma ghrelin levels in two groups. This is the first study investigating the DNA methylation of GHRL and GHSR genes in AUD.en_US
dc.description.sponsorshipAnkara Universitesien_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.doi10.1007/s11033-024-09585-4
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue1en_US
dc.identifier.pmid38771494en_US
dc.identifier.scopus2-s2.0-85193612178en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-024-09585-4
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14500
dc.identifier.volume51en_US
dc.identifier.wosWOS:001228895000001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGhrelinen_US
dc.subjectGrowth hormone secretagogue receptor type A1en_US
dc.subjectDNA methylationen_US
dc.subjectAlcohol use disorderen_US
dc.subjectAddictionen_US
dc.subjectDna Methylationen_US
dc.subjectGhrelinen_US
dc.subjectPromoteren_US
dc.subjectHypermethylationen_US
dc.subjectDependenceen_US
dc.subjectAppetiteen_US
dc.subjectNicotineen_US
dc.subjectRegionen_US
dc.titleA case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorderen_US
dc.typeArticleen_US

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