Gram negatif bakterilerde beta-laktamaz varlığının ve antibiyotik duyarlılıklarının araştırılması
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Tarih
2011
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info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada, hastanemizde izole edilen bazı Gram negatif bakterilerin beta-laktamaz üretimi ve antibiyotik duyarlılıklarının araştırılması amaçlanmıştır. Yöntem: İndüklenebilir beta-laktamaz varlığı direk indüksiyon testiyle, genişlemiş spektrumlu beta-laktamaz varlığı çift disk sinerji testiyle, in-vitro duyarlılık testleri ise; Clinical and Laboratory Standards Institute önerileri doğrultusunda, Kirby-Bauer disk difüzyon yöntemi ile araştırılmıştır. Bulgular: İncelenen suşların % 2’sinde indüklenebilir beta-laktamaz, % 8’inde genişlemiş spektrumlu beta-laktamaz varlığı saptanmıştır. Çalışmamızda, imipeneme dirençli suş saptanmazken, ampisilin, amoksisilin/klavulanik asit ve siprofloksasine karşı direnç oranı yüksek bulunmuştur. Sonuç: Hastanemizde büyük risk oluşturmayan infeksiyonların ampirik tedavisinde amikasin, gentamisin, sefoperazon/sulbaktam ve piperasilin/tazobaktamın karbapenemlere alternatif olarak kullanılabileceği düşünülürken; ampisilin, amoksisilin/klavulanik asit, siprofloksasinin bunun için uygun olmadığı görülmüştür
Objective:. In this study, beta-lactamase production and antibiotic susceptibility in some Gramnegative bacteria isolated in our hospital were investigated. Methods: The presence of inducible beta-lactamase by direct induction test, the presence of extended-spectrum beta-lactamase by double-disk synergy method were investigated. In-vitro susceptibility tests were performed by disk diffusion method, in the direction of the Clinical and Laboratory Standards Institute recommendations. Results: The rate of inducible beta-lactamase was found as 2 %, and the rate of extendedspectrum beta-lactamase as 8 %. Imipenem was the most effective antimicrobial. The rates of resistance to ampicillin, amoxicillin-clavulanic acid and ciprofloxacin were very high. Conclusion: Although, amikacin, gentamicin, sefoperazon-sulbactam, piperacillin-tazobactam can be used as alternatives to carbapenems in empirical treatment of nonserious infections in our hospital, ampicillin, amoxicillin-clavulanic acid and ciprofloxacin are not suitable for this purpose.
Objective:. In this study, beta-lactamase production and antibiotic susceptibility in some Gramnegative bacteria isolated in our hospital were investigated. Methods: The presence of inducible beta-lactamase by direct induction test, the presence of extended-spectrum beta-lactamase by double-disk synergy method were investigated. In-vitro susceptibility tests were performed by disk diffusion method, in the direction of the Clinical and Laboratory Standards Institute recommendations. Results: The rate of inducible beta-lactamase was found as 2 %, and the rate of extendedspectrum beta-lactamase as 8 %. Imipenem was the most effective antimicrobial. The rates of resistance to ampicillin, amoxicillin-clavulanic acid and ciprofloxacin were very high. Conclusion: Although, amikacin, gentamicin, sefoperazon-sulbactam, piperacillin-tazobactam can be used as alternatives to carbapenems in empirical treatment of nonserious infections in our hospital, ampicillin, amoxicillin-clavulanic acid and ciprofloxacin are not suitable for this purpose.
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