Investigation of PIC1 (permease in chloroplasts 1) gene's role in iron homeostasis: bioinformatics and expression analyses in tomato and sorghum

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dc.authoridAkbudak, M. Aydin/0000-0002-1397-4678
dc.contributor.authorFiliz, Ertugrul
dc.contributor.authorAydin Akbudak, M.
dc.date.accessioned2021-12-01T18:48:40Z
dc.date.available2021-12-01T18:48:40Z
dc.date.issued2020
dc.department[Belirlenecek]en_US
dc.description.abstractIron (Fe) is a crucial micronutrient in plant metabolism; thus, iron homeostasis is critical for plant development. Permease in chloroplast 1 (PIC1) is the first protein determined in the chloroplast playing a role iron homeostasis. In the present study, the PIC1 gene was investigated at a genome-wide scale in four plant genomes; Arabidopsis, tomato, maize and sorghum. Based on the gene ontology database, 21 GO terms were found related to the PIC1 gene, most of which were involved in iron hemostasis and transport. The digital expression data revealed that the expression of the majority of PIC1 genes (62.5%) in Arabidopsis decreased under abiotic stress conditions. Expression profiles of tomato PIC1 (SlPIC1) and sorghum PIC1 (SbPIC1) genes were also analyzed under salt and drought stress conditions using Real Time-quantitative PCR (RT-qPCR). Our wet-lab studies demonstrated that the SbPIC1 gene was down-regulated under salt and drought stresses in all tissues, while SlPIC1 was up-regulated in all but root tissue under drought stress. Some structural variations were detected in predicted 3D structures of PIC1 proteins and the structural similarity values varied between 0.23 and 0.35. Consequently, these results may contribute to the understanding of the PIC1 gene in iron transport and homeostasis in plants.en_US
dc.identifier.doi10.1007/s10534-019-00228-x
dc.identifier.endpage44en_US
dc.identifier.issn0966-0844
dc.identifier.issn1572-8773
dc.identifier.issue1en_US
dc.identifier.pmid31802376en_US
dc.identifier.scopus2-s2.0-85076043371en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage29en_US
dc.identifier.urihttps://doi.org/10.1007/s10534-019-00228-x
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10581
dc.identifier.volume33en_US
dc.identifier.wosWOS:000500604000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiometalsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIron homeostasisen_US
dc.subjectPIC1en_US
dc.subjectDroughten_US
dc.subjectSalt stressen_US
dc.subjectGene expressionen_US
dc.subjectBioinformaticsen_US
dc.subjectStructure Predictionen_US
dc.subjectTransit Peptidesen_US
dc.subjectTransporten_US
dc.subjectPhosphatidylglycerolen_US
dc.subjectPhosphorylationen_US
dc.subjectBiosynthesisen_US
dc.subjectEvolutionen_US
dc.subjectProteinsen_US
dc.subjectMovementen_US
dc.subjectAncienten_US
dc.titleInvestigation of PIC1 (permease in chloroplasts 1) gene's role in iron homeostasis: bioinformatics and expression analyses in tomato and sorghumen_US
dc.typeArticleen_US

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