Gamma İnterferon Salınım Testi İle Latent Tüberküloz İnfeksiyonunun Araştırılması
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Dosyalar
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Duzce Univ
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Tüberkülozun ilk infeksiyonu genellikle gizli kalmakta ve vücut savunma
mekanizmaları tarafından sınırlandırılmaktadır. Vücut direncinde zayıflama olursa bu
infeksiyon aktif hale gelebilmektedir. Latent infeksiyon radyolojik ve bakteriyolojik olarak
tanınamamaktadır. Bu infeksiyonun erken tespit edilmesi günümüzde pek çok hastalıkta
kullanılan immunosupresif tedaviler öncesinde gerekmektedir. Ayrıca aile içi tüberküloz
geçişinin ve küçük çocuklardaki infeksiyonun gösterilmesinde önemlidir.
Gereç ve Yöntem: Çalışmaya, 20.02.2016-15.03.2017 tarihleri arasında Düzce Üniversitesi
Tıp Fakültesi Hastanesi’nin servis, yoğun bakım ve polikliniklerinden tüberküloz şüphesi
olan hastalar, sağlık personeli ve verem savaş dispanseri personeli olan toplam 76 kişi dahil
edilmiştir. Latent infeksiyonun gösterilmesinde gama interferon salınım testi “QuantiferonTb Gold ELISA testi (QIAGEN, Almanya)” kullanılmıştır.
Bulgular: Toplam 19 (% 25) kişide test pozitif olarak saptanmıştır. Pozitiflik saptanan 19
kişinin 8 (% 42)’ i akciğer tüberkülozu tanısı, 4 (% 21)’ü akciğer dışı tüberküloz ön tanısı ve
7 (% 37)’si tüberkülozlu hastalarla temas etmiş olması nedeniyle çalışmaya alınmıştır.
Pozitiflik saptanan kişilerin ikisi (% 10.5) akciğer tüberkülozu, biri (% 5.2) tüberküloz
lenfadenit, biri (% 5.2) ise tüberküloz menenjit tanısı almıştır. Verem savaş dispanserinden
çalışmaya alınan sağlık personelinin hepsinde test pozitif bulunmuştur. Son olarak,
hastanemiz mikrobiyoloji laboratuvarında çalışan 19 kişiden sadece birinde test pozitif
bulunmuş olup onun da ailesinde tüberküloz öyküsü olduğu öğrenilmiştir.
Sonuç: Quantiferon Tb gold ELİSA testinin tüberkülozlu hastalarla sürekli temasta olan
sağlık çalışanları başta olmak üzere, tüm sağlık çalışanlarında; ayrıca aktif tüberküloz
infeksiyonu düşünülüp kültür sonucu çıkıncaya kadar hastanın tedavisinin erken
planlanmasının gerektiği durumlarda uygulanabilecek bir yöntem olduğu düşünülmüştür.
_The initial infection of tuberculosis is usually confidential and limited by body defense mechanisms. If there is weakening of the body resistance, this infection may become active. Latent infection can not be recognized as radiological and bacteriologically. Early detection of infection is necessary before immunosuppressive therapies are used in many diseases today. It is also important to demonstrated to transmission of tuberculosis from family members and presence of infections in small children. Methods_ A total of 76 people were included in the study between 20.02.2016-15.03.2017 with patients with tuberculosis suspicion at Duzce University Faculty of Medicine Hospital's intensive care unit and outpatient clinics, health personel and tuberculosis dispensary personel. Gamma interferon release assay _Quantiferon-Tb Gold ELISA test (QIAGEN, Germany)_ was used to demonstrate latent infection. Results_ A total of 19 (25%) subjects had a positive test. Of the 19 patients who were positive, 8 (42%) had a pulmonary tuberculosis pre-diagnosis, 4 (21%) of them had an extrapulmonary tuberculosis pre-diagnosis and 7 (37%) had contact with tuberculosis patients. Two of the patients (10.5%) were positive for lung tuberculosis, one (5.2%) were tuberculous lymphadenitis and one (5.2%) were tuberculous meningitis. All health personel who were taken to the TB dispensary were found to be positive. Finally, only one of the 19 patients have been working at microbiology laboratory of our hospital who determinate positive test results, and he had a family story of tuberculosis. Conclusion_ The Quantiferon Tb gold ELISA test was thought to be a method that could be applied to all healthcare workers, especially healthcare workers who are constantly in contact with patients with tuberculosis. It is also considered appropriate to use of the patient with suspected active tuberculosis infection until culture end_
_The initial infection of tuberculosis is usually confidential and limited by body defense mechanisms. If there is weakening of the body resistance, this infection may become active. Latent infection can not be recognized as radiological and bacteriologically. Early detection of infection is necessary before immunosuppressive therapies are used in many diseases today. It is also important to demonstrated to transmission of tuberculosis from family members and presence of infections in small children. Methods_ A total of 76 people were included in the study between 20.02.2016-15.03.2017 with patients with tuberculosis suspicion at Duzce University Faculty of Medicine Hospital's intensive care unit and outpatient clinics, health personel and tuberculosis dispensary personel. Gamma interferon release assay _Quantiferon-Tb Gold ELISA test (QIAGEN, Germany)_ was used to demonstrate latent infection. Results_ A total of 19 (25%) subjects had a positive test. Of the 19 patients who were positive, 8 (42%) had a pulmonary tuberculosis pre-diagnosis, 4 (21%) of them had an extrapulmonary tuberculosis pre-diagnosis and 7 (37%) had contact with tuberculosis patients. Two of the patients (10.5%) were positive for lung tuberculosis, one (5.2%) were tuberculous lymphadenitis and one (5.2%) were tuberculous meningitis. All health personel who were taken to the TB dispensary were found to be positive. Finally, only one of the 19 patients have been working at microbiology laboratory of our hospital who determinate positive test results, and he had a family story of tuberculosis. Conclusion_ The Quantiferon Tb gold ELISA test was thought to be a method that could be applied to all healthcare workers, especially healthcare workers who are constantly in contact with patients with tuberculosis. It is also considered appropriate to use of the patient with suspected active tuberculosis infection until culture end_
Açıklama
WOS: 000419631500010
Anahtar Kelimeler
Diagnosis, Gamma Interferon Release Test, Latent Tuberculosis
Kaynak
Konuralp Tip Dergisi
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
9
Sayı
3
