Modulation of Brain-derived Neurotrophic Factor and Nerve Growth Factor Gene Expression in the Hippocampus and Cortex Regions of Rats with Penicillin-induced Experimental Epilepsy: The Effect of Adenosine Triphosphate-sensitive Potassium Channels
Küçük Resim Yok
Tarih
2025
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wolters Kluwer Medknow Publications
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective:The objective is to investigate the effect of adenosine triphosphate (ATP)-sensitive potassium (KATP) channels on brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) gene expression in the hippocampus and cortex regions using a penicillin-induced epilepsy model. Materials and Methods:The study included four groups, control (C), epilepsy (E), epilepsy-opener (E-O), and epilepsy-blocker (E-B), and three subgroups, 1st day, 4th day, and 8th day after the seizure. The hippocampus and cortex were dissected from all animals and BDNF and NGF messenger ribonucleic acid (mRNA) expression levels were quantified using quantitative polymerase chain reaction (qPCR). Results:The relative gene expression of the NGF gene evaluated in hippocampal tissues was found to be significantly higher in the epilepsy 1st-day group compared with the C group (P < 0.05). The relative gene expression of the NGF gene in cortex tissues was found to be significantly higher in the epilepsy 8th-day group compared with the C group (P < 0.05). The relative gene expression of the BDNF gene in hippocampal tissues was also significantly higher in the epilepsy 1st-day group compared with the C group (P < 0.05). BDNF gene expression levels in the cortex region were significantly higher in the E group on the 8th day, in the E-O group on the 1st, 4th, and 8th days, and in the E-B group on the 1st day; BDNF gene expression level was also significantly higher in the E-B group on the 8th day compared with the C group (P < 0.05). Conclusion:KATP channels play a modulatory role in the expression levels of BDNF and NGF genes in the cortex over an extended period of 8 days, parallel to their efficacy observed in the hippocampus on day 1. These mechanisms need to be thoroughly investigated and emphasized due to their importance.
Açıklama
Anahtar Kelimeler
Adenosine triphosphate-sensitive potassium channels, brain-derived neurotrophic factor, epilepsy, nerve growth factor
Kaynak
Neurological Sciencesand Neurophysiology
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
42
Sayı
1
