Yazar "Çetinkaya, Ayhan" seçeneğine göre listele
Listeleniyor 1 - 15 / 15
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe AMELIORATING EFFECT OF HAWTHORN (CRATAEGUS OXYACANTHA) AND PHYSICAL EXERCISE ON ACUTE PENICILLIN INDUCED SEIZURES IN GERBILS(African Networks Ethnomedicines, 2016) Çakır, Serkan; Orallar, Hayriye; Çetinkaya, Ayhan; Kayacan, Yıldırım; Önal, Ali Can; Yıldırım, Arzu; Okur, NezihBackground: The aim of the present study was to assess the effect of Hawthorn (Crataegus oxyacantha) and physical activity. We studied its effect on penicillin induced epilepsy. in gerbils. Materal and Methods: Epilepsy was induced by administration of peniciline G (500 IU, ip). The gerbils were divided randomly in four groups (6 animals per each group) and studied as described below: 1) Control group 2) Exercise group (30 min/each day for 8 weeks) (Eg) 3) Extract group, 50mg/kg/day/animal in 1 ml saline, 3 h prior to exercise (Exe) 4) Exercise+ Extract + (Exe+ Ex). The severity of epilepsy was observed and recorded. Results: The means of latencies (Mean +/- SE) were 236 +/- 45, 369 +/- 36, 386 +/- 58 and 433 +/- 37 ms in groups of control, Exe, Ex, and Exe+ Ex respectively. The mean spike latency significantly (P= 0,033 F= 3,560) decreased in Exe, Ex and Exe+ Ex when compared control. Although spike frequency significantly (P< 0.05) diminished in groups of Exe and Ex, no significant decrease was observed in control and Exe+ Ex. Similar trend was seen for amplitude values. Spike amplitude values were determined to be significantly (P< 0.05) lower than those of control and Exe+ Ex. Conclusion: Crataegus oxyacantha extract has shown positive affect to ameliorate on some seizure parameters in this study. However, further more advanced physiologic and neurochemical studies are required to determine the mechanisms involved.Öğe Deneysel Nörolojik Hastalık Modellemelerinde Anestezi Uygulamaları(Düzce Üniversitesi, 2024) Çetinkaya, Ayhan; Çelik, HümeyraBeynin hangi moleküler mekanizmalar kullanarak nörolojik hastalıkların oluştuğu ve nasıl tedavi edilebileceği kapsamındaki sinir bilim, multidisipliner yaklaşımlar ile erken tanı ve yeni tedavi geliştirmek üzere yüksek bütçeli araştırmaların konusu olarak güncelliğini korumaktadır. Bu amaçla nörolojik in vivo deneysel modellerde uygun modeli doğru metot ve anestezi ile oluşturmak doğru sonuçları elde etmenin ve hayvan refahını sağlamanın en önemli basamaklarıdır. Kemirgenlerde anesteziyi yönetmek adına anesteziklerin fizyolojik özelliklerini tanımak ve risklerine hakim olmak deneysel prosedürleri güçlendirecektir. Enjekte edilebilir anesteziklerden ketamin, ksilazin ve pentobarbital genel anestezi için kısa cerrahi prosedürlerde en sık tercih edilen ajanlardır. İzofluran ve sevofluran sıvı olduklarından bir vaporizer ile verilen inhaler anesteziklerdendir. İnhale anesteziklerin hızlı indüksiyon ve hızlı çekilme gibi önemli avantajları sinirbilim çalışmalarında inhaler anestezikleri öne çıkarmaktadır. Bu derlemede kemirgenlerde sık kullanılan anestezik ajanların özellikleri, kullanım şekilleri ve hangi modelde tercih edildiğinden bahsedilecektir. Bu amaçla epilepsi, Alzeimer hastalığı, iskemi-reperfüzyon hasarı, travmatik beyin hasarı, iskemik inme, deneysel otoimmun ensefalomyelitis, oftalmik cerrahi prosedürler ve yan etkileri gibi hayvan modellerinde uygun anesteziklerin seçimi gözden geçirilecektir.Öğe Effect of Acute and Chronic Ellagic Acid Administration on Penicillin-induced Epileptiform Activity in Rats(Wiley, 2017) Beyazçiçek, Ersin; Ankaralı, Seyit; Beyazçiçek, Özge; Ankaralı, Handan; Çetinkaya, Ayhan…Öğe The Effect of Rose Oil on Penicillin-Induced Epileptiform Activity in Rats: An Electrophysiological Study(Duzce Univ, 2018) Ankaralı, Seyit; Beyazçiçek, Ersin; Kılınç, Erkan; Beyazçiçek, Özge; Özkan, Kayhan; Çetinkaya, Ayhan; Ankaralı, HandanObjective: Rose oil (from Rosa damascene) has several effects which are analgesic, antispasmodic, antioxidant and neuroprotective role. Its antiepileptic effect has not been yet studied enough. In the present study, it was aimed to investigate acute effects of rose oil on the epileptiform activity in penicillin-induced epilepsy model in rats. Methods: Forty-two male Wistar rats weighing 230 to 260 g were divided into six groups with seven rats in each group. Control (+Penicillin), RO alone, Diazepam, and different doses of Rose oil including 100, 500 and 1000 mg/kg. Rats were pretreated with different doses of RO 30 min prior to penicillin treatment. Electrocorticogram recordings were taken from each animal for 2 hours after penicillin treatment. Results: Only the dose of 100 mg/kg of rose oil reduced significantly epileptic spike-wave frequency of epileptiform activity. However, comparing in terms of latency and spike-wave amplitude of epileptiform activity, there were no significant difference between the groups. Conclusions: In conclusion, acute administration of rose oil reduces spike-wave frequency of penicillin-induced epileptiform activity in rats. Therefore, these findings indicate that rose oil has antiepileptic effects.Öğe The effect of vortioxetine on penicillin-induced epileptiform activity in rats(Assoc Arquivos Neuro- Psiquiatria, 2019) Ogün, Muhammed Nur; Çetinkaya, Ayhan; Beyazçiçek, ErsinVortioxetine is a multimodal antidepressant agent that modulates 5-HT receptors and inhibits the serotonin transporter. It is indicated especially in cases of major depressive disorder related to cognitive dysfunction. There are many studies investigating the effects of antidepressants on the seizure threshold and short-term epileptic activity. However, the effect of vortioxetine on epileptic seizures is not exactly known. Our aim was to investigate the effects of vortioxetine on penicillin-induced epileptiform activity. Twenty-seven Wistar rats were divided into three groups: sham-control group, positive control group (diazepam), and vortioxetine group. After a penicillin-induced epilepsy model was formed in each of the three groups of animals, 0.1 ml of saline was administered to the control group, 0.1 ml (10 mg/kg) vortioxetine was administered in the vortioxetine group, and 0.1 mL (5 mg/kg) of diazepam was administered in the positive control group, intraperitoneally. The epileptic activity records were obtained for 120 minutes after the onset of seizure.There was no significant difference in spike wave activity between the vortioxetine and diazepam groups, whereas this was significantly reduced in the vortioxetine group compared with the controls. The administration of vortioxetine at a dose of 10 mg/kg immediately after the seizure induction significantly decreased the spike frequencies of epileptiform activity compared with the control group. No significant difference was found between the vortioxetine and positive controls.This study showed that vortioxetine reduces the number of acutely-induced epileptic discharges. Vortioxetine may be an important alternative for epileptic patients with major depressive disorder-related cognitive dysfunction.Öğe The Effects of Nigella Sativa Extract and Chronic Exercise Application on Penicillin-induced Epilepsy Model in Mongolian Gerbils(Wiley-Blackwell, 2016) Çetinkaya, Ayhan; Demir, Şerif; Orallar, Hayriye; Kayacan, Yıldırım; Çakır, Serkan; Yıldırım, Arzu; Beyazçiçek, Ersin…Öğe Effects of the ATP-dependent K (+)-channel effectors pinacidil and glibenclamide on liver tissue in an experimental model of epilepsy: A histopathological study(2022) Çetinkaya, Ayhan; Demir, Şerif; Düzcü, Selma Erdoğan; Orallar, HayriyeAim: It is known that most of the antiepileptic drugs have negative effects on the liver. Pinacidil is a nonselective opener of KATP channels, including the plasma membrane and mitochondria. Glibenclamide is an ATP -dependent K channel blocker ensuring the intake of calcium. Our aim in this experimental study was to examine the effects of pinacidil and glibenclamide on the liver tissue of rats with focal epilepsy. Method: Sixty male Sprague Dawley rats (2-4 months old, 200-250 gr) were used in the study. The rats were divided into 4 groups, 15 in each group. The groups were divided into control group, penicillin group, penicillin + pinacidil group and penicillin + glibenclamide group. The craniums of the rats in the control group were opened and normal saline was given; Penicillin (2 ?l 500 IU) was intracortically administered to other groups and an experimental epilepsy model was created. At the end of the study, liver tissue of rats was taken and evaluated in terms of vacuolar degeneration, lymphocyte infiltration, vascular congestion, sinusoidal dilatation, necrosis, and Kupffer cell proliferation, radial alignment of hepatic cords, central vein and portal vein dilatation in hepatocytes. Results: Venous congestion, cytoplasmic vacuolization, Kupffer cell proliferation, portal vein dilatation and necrosis were distinct in the group to which pinacidil was administered, and distortion was present in the radial sequence (p<0.001). In addition, inflammation, venous congestion and hepatocyte necrosis were found to be lower in the glibenclamide given group compared to the control group (p<0.001). Conclusion: It can be suggested that pinacidil treatment caused negative results in liver histopathological parameters, whereas glibenclamide was more protective by reducing inflammation, venous congestion and hepatocyte necrosis.Öğe Gender Specificity of Genistein Treatment in Penicillin-Induced Epileptiform Activity in Rats(Springer, 2016) Bahadır, Anzel; Demir, Şerif; Orallar, Hayriye; Beyazçiçek, Ersin; Çetinkaya, Ayhan; Ankaralı, Seyit; Ankaralı, HandanWe investigated gender-dependent differences of genistein (isoflavone phytoestrogen) treatment in a penicillin-induced experimental epilepsy rat model. Twenty-eight adult Wistar Albino rats (14 females and 14 males) were devided into four groups, control and genistein-treatmed males and females. Genistein (100 mu g/kg, i.p) or saline was given during 15 days before the electrocorticography (ECoG) recordings. The epileptiform activity was induced by penicillin G potassium salt (500 IU) injections into the left somatomotor cortex. Significant differences among the groups were found in the latency to onset of epileptiform activity. This value in the female control group was significantly longer than the latencies in the male control, male genistein, and female genistein groups (respectively, P = 0.002, 0.015, and 0.032). There were no significant differences regarding the spike/wave frequencies and amplitudes in epileptiform activity between female/male genistein and control groups within all observation intervals (P > 0.05). Thus, genistein exerts a proconvulsant effect in the penicillin-induced epilepsy model, and the effect demonstrates the clear gender specificity related to the specificity of hormonal backgrounds in males and females.Öğe Investigation of Anxiolytic Effects of Meprobamate for Pharmaceuticals Determination of a New Derivative of Mepronarilmate Composes(Wiley-Blackwell, 2016) Türkoğlu, Şule Aydın; Orallar, Hayriye; Ordu, Öznur Demir; Çetinkaya, Ayhan; Coşkun, Hamit; Ayaz, Erol…Öğe Mogolistan gerbillerinde çörek otu (nigella sativa) ekstraktının ve kronik egzersiz uygulanmasının penisilin modeli deneysel epilepsi üzerine etkileri(Düzce Üniversitesi, 2016) Çetinkaya, Ayhan; Demir, Şerif; Türkoğlu, Şule AydınEpilepsi, tekrarlayan nöbetlerle karakterize, insan yaşamını mental ve fiziksel olarak olumsuz etkileyen en yaygın sinir sistemi hastalıklarından birisidir. Eksitatör ve inhibitör mekanizmalar arası dengenin bozulması sonucu oluşan senkronize deşarjlarla karakterizedir. Bu çalışmanın amacı, moğolistan gerbillerinde çörek otu (Nigella sativa) ekstraktı ve koşubandı egzersizi uygulamalarının penisilin G ile oluşturulan epilepsi modeli üzerindeki etkilerinin elektrofizyolojik olarak araştırılması ve oksidatif stres üzerine olan etkilerinin incelenmesidir. Bu çalışmada 40 adet yetişkin erkek Moğolistan gerbili kullanıldı. Hayvanlar kontrol (saline) grubu, kontrol (penisilin) grubu, egzersiz grubu, çörek otu grubu ve çörek otu-egzersiz grubu olmak üzere toplam 5 gruba ayrılmıştır. Egzersiz gruplarına iki ay süre ile haftada beş gün ilk toplamda 30 dak. koşu bandı egzersizi uygulanmıştır. Çörek otu gruplarına ise iki ay süre ile haftada 5 gün gavaj yolu ile 50 mg/kg dozda 0,2 ml hacimde çörek otu ekstraktı verilmiştir. İki aylık egzersiz ve gavaj uygulamalarından sonra epileptiform aktivite, intrakortikal penisilin (500 IU/ 2,5 ?l) uygulanmasıyla oluşturulmuş, power lab sistemi ile her hayvandan 120 dak.'lık elektrokortikografi (ECoG) kayıtları alınarak analizi yapılmıştır. Kontrol (saline) grubunda herhangi epileptik aktiviteye rastlanmamıştır. Çalışmada elde edilen bulgulara göre çörek otu ve egzersiz ayrı ayrı uygulandığında, epileptiform aktivitenin başlama zamanını geciktirdiği ancak çörek otu-egzersiz kombinasyonunun daha fazla etkili olduğu görülmüştür. Moğolistan gerbillerinde çörek otu ve egzersiz uygulaması hem nöbeti geciktirmiş hem de diken dalga sayısını azaltmıştır. Epileptiform aktivite genlikleri değerlendirildiğinde bazı periyotlar hariç kontrol grubu ile diğer gruplar arasındaki fark anlamlı bulunmuştur. Sonuç olarak, çörek otu ve egzersizin birlikte uygulanmasının ilk epileptiform aktivitenin başlama zamanını geciktirdiği, epileptiform aktivitenin diken dalga sıklığını ve genliğini azalttığı gösterilmiştir. Bu durum epilepsili hastaların çörek otu tüketimi ile birlikte egzersiz yapmaların yararlı olabileceğini düşündürmektedir.Öğe Penicillin-Induced Epileptiform ECoG Activity in Gerbils: Effects of Physical Exercise and a Diospyros kaki Extract(Springer, 2016) Kayacan, Yıldırım; Bahadır, Anzel; Çetinkaya, Ayhan; Orallar, Hayriye; Çakır, S.; Beyazçiçek, Ersin; Yıldırım, Arzu BirinciMongolian gerbils (28 males) were divided into four groups, control (C), treadmill-exercised (Ex), treated with the extract of Diospyros kaki (Dk), and exercised plus treated with the Dk extract (Ex+Dk). Animals of the respective groups were running-exercised for 30 min per day during 8 weeks, and the Dk extract (dose 20 mg/kg) was given by gavage during five days per week within the same period. After the treatment and exercise period, an epilepsy model was produced by penicillin G injection (500 IU) into the left somatomotor cortex, and the electrocorticogram (ECoG) was recorded during 120 min. The mean frequency of spike/wave complexes was significantly smaller in the Ex and Ex+Dk groups from the 65th min of the observation period and, in the Dk group, from the 75th min than the respective value in the C group (P < 0.01, P < 0.05, and P < 0.01, respectively). The differences in the amplitude values and latency to onset of the spike/wave events among all groups did not reach the significance level (P > 0.05). Thus, both the running exercise and Dk extract applications inhibit penicillin-induced epileptiform activity by altering the spike/wave frequency or severity of seizures observed in ECoG recordings. Further studies are needed to determine the effects of physical activity of different intensities and forms and to analyze the active compounds in the Dk extract.Öğe Protective effect of oxymatrine against renal ischemia/reperfusion injury in rats(Comenius Univ, 2017) Hülya, Öztürk; Çetinkaya, Ayhan; Yılmaz, FahriBACKGROUND: Renal ischemia/reperfusion (I/R) injury is a common cause of acute kidney injury. The pathologic mechanisms underlying renal I/R injury are complicated, involving reactive oxygen species, necrosis, cell apoptosis, and inflammation, but the exact mechanisms remain unclear. OBJECTIVES: This study aimed to investigate the effect of oxymatrine (OMT) on renal I/R injury and its underlying mechanism. METHODS: Thirty male Sprague Dawley rats were randomly allocated to three groups (n = 10): the sham-control group, the renal I/R-untreated (I/R-untreated) group, and the I/R-OMT group. Renal I/R injury were induced by clamping the left renal artery for 45 min followed by 24 h of reperfusion. At 10 min before reperfusion, the rats in the I/R-OMT-treated group rats received an intravenous injection of 40 mg/kg OMT. Renal function and histological changes were compared and the relevant parameters of oxidative stress and inflammation were detected. RESULTS: Oxymatrine pretreatment significantly decreased the level of renal dysfunction, attenuated the renal histological changes, the levels of reactive oxygen species production in renal tissue upon I/R. Additionally, OMT pretreatment could further activate the serum antioxidant enzyme activities. CONCLUSION: The beneficial effects of OMT were likely mediated by the inhibition of lipid peroxidation and the increase in endogenous antioxidant activity. The results of this study indicate that oxymatrine may represent a potent anti-oxidant drug to protect the kidney against I/R injury (Fig. 5, Ref. 29). Text in PDF www.elis.sk.Öğe Ratlarda Amiloid Beta1-42 İle Oluşturulan Deneysel Alzheimer Modelinde Tiyol Disülfit Homeostazisi(2020) Çetinkaya, AyhanAmaç: Bu çalışmanın amacı, amiloid beta 1-42 enjekte edilerek Alzheimer modeli oluşturulan ratlarda, oksidan veantioksidan dengenin yeni bir oksidatif stres belirteci olan dinamik tiyol disülphide homeostazisinin, serum total tiyol,natif tiyol, ve disülfit seviyelerinin araştırılması ve disulfit/total tiyol ve disulfit/natif tiyol oranlarınındeğerlendirilmesidir.Gereç ve Yöntemler: Bu deneysel çalışma 28 rat üzerinde gerçekleştirildi. Denekler her grupta 14 adet olacak şekilde,Alzheimer ve kontrol grubu olarak ikiye ayrıldı. Amiloid beta 1-42, enjeksiyon için bir hafta inkubatörde bekletilerektoksititesi artırıldı. Alzheimer grubunda yer alan hayvanlara bilateral 4 µl amiloid beta 1-42 enjekte edilerek deneyselAlzheimer modeli oluşturuldu. 10 gün beklenildikten sonra tüm hayvanlardan 90/10 mg/kg ksilazine/ketamin anestezisialtında intra kardiyak alınan kan numuneleri ile laboratuvarda TDH parametreleri çalışıldı (nativ tiyol, total tiyol,disülfid, disulfit/total tiyol oranı ve disulfit/natif tiyol oranı) ve bu parametreler gruplar arasında karşılaştırıldı.Bulgular: Çalışma ve kontrol grupları karşılaştırıldığında, serum total tiyol düzeyleri (469,85±30,65 ve 558,00±23,46)ile serum disülfid düzeyleri (182,57±15,74 ve 224,85±11,95), alzheimer modeli oluşturulan grupta kontrol grubuna göreistatistiksel olarak anlamlı bir şekilde azalma görülürken (p<0,05) serum natif tiyol düzeylerinde (104,71±8,17 ve108,28±9,71) ise anlamlı bir fark bulunamamıştır(p>0,05).Sonuç: İlgili çalışma, deneysel olarak oluşturulan Alzheimer modellemelerinde serumda dinamik tiyol-disülfidhomeostazını değerlendiren ilk araştırmadır. Çalışmamızın sonuçları TDH’ın deneysel alzheimer modellemelerinde,oksidatif stres mekanizmalarının değerlendirilmesinde ucuz ve kolay yeni bir markerı olabileceğini düşündürmektedir.Öğe Therapeutic Effects of Carvacrol on Beta-Amyloid-Induced Hippocampal Neurotoxicity, Oxidative Stress, and Memory Impairment in a Rat Model of Alzheimer's Disease(Wiley, 2022) Topkara, Kübra Çelik; Kılınç, Erkan; Çetinkaya, Ayhan; Saylan, Asıihan; Demir, Şerif[Bastract Not Available]Öğe Therapeutic effects of carvacrol on beta-amyloid-induced impairments in in vitro and in vivo models of Alzheimer's disease(Wiley, 2022) Topkara, Kübra Çelik; Kılınç, Erkan; Çetinkaya, Ayhan; Saylan, Asıihan; Demir, ŞerifDue to the complex nature of Alzheimer's disease (AD), it is important to investigate agents with multiple effects in the treatment of AD. Carvacrol possesses anti-acetylcholinesterase, anti-oxidant, and neuroprotective properties. We therefore investigated therapeutic effects of carvacrol on cell viability, oxidative stress, and cognitive impairment in A beta 1-42-induced in vitro and in vivo models of AD. SH-SY5Y cells differentiated into neurons by retinoic acid were pretreated with carvacrol or galantamine before A beta 1-42 administration. For in vivo experiments, a rat model of AD was established by bilateral intrahippocampal injection of A beta 1-42. The groups received 1% DMSO, carvacrol, or galantamine intraperitoneally twice a day (morning and afternoon) for 6 days. Cell viability was determined using MTT and LDH tests. Learning and memory functions were assessed using a passive-avoidance test. Oxidant-antioxidant parameters (MDA, H2O2, SOD, and CAT) and Tau, A beta 1-40, and A beta 1-42 peptide levels in in vitro supernatant or in vivo serum and hippocampal samples were measured using ELISA. Carvacrol increased cell viability and exhibited a protective effect against oxidative stress by preventing A beta 1-42-induced cytotoxicity, LDH release, and increments in MDA and H2O2 levels in vitro. Additionally, it improved memory impairment by reversing A beta 1-42-induced changes on passive-avoidance test. Carvacrol ameliorated A beta 1-42-induced increments in MDA and H2O2 levels in in vitro supernatant and in vivo hippocampal samples. However, none of the treatments changed in vitro SOD and Tau-peptide levels, or in vivo serum levels of MDA, H2O2, SOD, CAT, Tau peptide, A beta 1-40, or A beta 1-42. Our results suggest that multi-target pharmacological agent carvacrol may be promising in treatment of AD by preventing beta-amyloid-induced neurotoxicity, oxidative stress, and memory deficits.
