Production of autotetraploid plants by in vitro chromosome engineering in Allium hirtifolium

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dc.authorscopusid55797332400
dc.authorscopusid16836972700
dc.authorscopusid15832676900
dc.authorscopusid36102637400
dc.contributor.authorFarhadi, Nasrin
dc.contributor.authorPanahandeh, Jaber
dc.contributor.authorMotallebi-Azar, Alireza
dc.contributor.authorMokhtarzadeh, Sam
dc.date.accessioned2023-07-26T11:54:31Z
dc.date.available2023-07-26T11:54:31Z
dc.date.issued2023
dc.departmentDÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.description.abstractPersian shallot has been propagated vegetatively through daughter bulbs and has a narrow genetic base. In the present study, to create genetic diversity in terms of ploidy level, the effects of in vitro polyploidization were investigated on morphological, physiological and phytochemical traits. Different colchicine treatments (0, 0.3%, 0.5% and 0.7% concentrations) for 24, 36 and 48 h and oryzalin treatments (0, 0.001%, 0.002% and 0.004% concentrations) for 4, 6 and 8 h were used. A sterile basal plate of bulbs was used for the induction of polyploidy in liquid and solid media. After obtaining plantlets, root tip chromosomes were counted for the determination of ploidy levels. Flow cytometry was performed to confirm the chromosome counting results. The regenerated diploid and tetraploid plantlets were transferred to ex vitro conditions. Colchicine and oryzalin were both successful in inducing polyploids and the polyploids induced were tetraploids and mixoploids. The highest induction of polyploidy was obtained in solid media using 0.5% (w/v) colchicine for 36 h (35.0% polyploidy induction) and in 0.001% (w/v) Oryzalin for 8 h (45.5% polyploidy induction). Differences in plantlet height and weight, bulblet weight, density, stomatal width and length, and chlorophyll content were observed between tetraploid and diploid plants. Chromosome duplication, as in the case of induced tetraploidy, significantly influenced the antioxidant content and enzyme activities. The results showed that total phenolic content, allicin, and antioxidant capacity were significantly higher in the tetraploid plantlets than that in the diploid plantlets. SRAP loci polymorphisms indicated that the 12 autotetraploid plants (with high allicin content) had different genotypes from the parental diploid plant. Besides, in vitro polyploidy induction not only duplicated chromosomes but also altered the DNA sequence in Allium hirtifolium. In vitro induction of tetraploids in A. hirtifolium can be a reliable way to obtain suitable plant material for breeding programs to generate new genotypic variations. © 2022 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).en_US
dc.description.sponsorshipUniversity of Tabrizen_US
dc.description.sponsorshipThis project was supported by the vice chancellery of research and technology of the Universityof Tabriz as the Ph D. thesis of first Author (N. Farhadi).en_US
dc.identifier.doi10.1016/j.hpj.2022.12.013
dc.identifier.issn2095-9885
dc.identifier.scopus2-s2.0-85146447742en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.hpj.2022.12.013
dc.identifier.urihttps://hdl.handle.net/20.500.12684/12855
dc.identifier.wosWOS:001090577000001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorMokhtarzadeh, Sam
dc.language.isoenen_US
dc.publisherKeAi Communications Co.en_US
dc.relation.ispartofHorticultural Plant Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz$2023V1Guncelleme$en_US
dc.subjectAllium hirtifoliumen_US
dc.subjectColchicineen_US
dc.subjectMolecular markeren_US
dc.subjectOryzalinen_US
dc.subjectPersian shalloten_US
dc.subjectPolyploidyen_US
dc.titleProduction of autotetraploid plants by in vitro chromosome engineering in Allium hirtifoliumen_US
dc.typeArticleen_US

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