Molecular characterization of the chitinase genes of native Bacillus thuringiensis isolates and their antagonistic activity against three important phytopathogenic fungi

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dc.authoridTEMIZGUL, RIDVAN/0000-0002-1033-7067
dc.authoridKaraborklu, Salih/0000-0003-4737-853X
dc.authoridAzizoglu, Ugur/0000-0003-1005-8444
dc.authorwosidTEMIZGUL, RIDVAN/AAL-7054-2021
dc.authorwosidKaraborklu, Salih/A-5033-2018
dc.contributor.authorAzizoglu, Zehra Busra
dc.contributor.authorYilmaz, Semih
dc.contributor.authorAzizoglu, Ugur
dc.contributor.authorKaraborklu, Salih
dc.contributor.authorTemizgul, Ridvan
dc.contributor.authorAyvaz, Abdurrahman
dc.date.accessioned2021-12-01T18:49:27Z
dc.date.available2021-12-01T18:49:27Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractBacillus spp. can promote the growth of plants and reduce plant disease incidence or severity by triggering induced systemic resistance to plant pathogens. In addition, bacteria of this genus are chitinase enzyme producers. Chitinase inhibits the growth of fungi by breaking down the chitin-containing cell wall of plant pathogenic fungi. In this study, 270 native Bacillus spp., isolated from various habitats in Kayseri and Adana, Turkey, were screened by PCR for the chitinase gene and 66 of them were found to have this gene. Nine Bacillus thuringiensis (Bt) isolates showing high insecticidal activity against insect pests in our previous studies were selected from 66 isolates containing the chitinase gene. Chitinases with different molecular weights ranging from similar to 40 to 113 kDa were determined by SDS-PAGE. To determine the antagonistic effects against plant pathogenic fungi (Fusarium oxysporum f.sp. niveum, Verticillium dahliae and Aspergillus niger), a dual culture assay was used with nine native and two standard strains of Bt, and radial growth inhibition was calculated as a percentage. Of all the tested isolates, SY33.3 showed the strongest antagonistic activity and thus, can be used as an effective biological control agent against plant pathogenic fungi.en_US
dc.description.sponsorshipErciyes University Scientific Project UnitErciyes University [FBD-08-540]en_US
dc.description.sponsorshipThis project was funded by Erciyes University Scientific Project Unit under the codes of FBD-08-540.en_US
dc.identifier.doi10.1007/s11756-021-00802-0
dc.identifier.endpage2755en_US
dc.identifier.issn0006-3088
dc.identifier.issn1336-9563
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-85107477678en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage2745en_US
dc.identifier.urihttps://doi.org/10.1007/s11756-021-00802-0
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10725
dc.identifier.volume76en_US
dc.identifier.wosWOS:000659808600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBten_US
dc.subjectBiological controlen_US
dc.subjectChitinaseen_US
dc.subjectPlant pathogenic fungien_US
dc.subjectStrainen_US
dc.subjectCloningen_US
dc.subjectPurificationen_US
dc.subjectDiseaseen_US
dc.titleMolecular characterization of the chitinase genes of native Bacillus thuringiensis isolates and their antagonistic activity against three important phytopathogenic fungien_US
dc.typeArticleen_US

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