Assessment of the Immunogenicity and Protective Aspects of a DNA Vaccine Targeting Crimean Congo Hemorrhagic Fever Virus Glycoprotein Gc
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2021
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info:eu-repo/semantics/openAccess
Özet
Aim: Crimean Congo Hemorrhagic Fever (CCHF) is a lethal, endemic infectious disease inhuman. For the preventive measures of the disease, there is currently no safe and efficient vaccine,widely for human use. Vaccine development for CCHF virus is an actively researched subject. Inthis study, we aimed to investigate the immunizing and protective potentials of the CCHF virussurface glycoprotein Gc that is delivered as a single antigen via a DNA based vaccine vector.Material and Methods: A DNA based vaccine targeting the immunogenic envelope glycoproteinGc of a CCHF virus isolate with Turkey origin (Ank2) was generated and its immunogenicity andprotective capability against lethal challenge in IFN?/?R-/- receptor knock out mice was assessed.Results: The developed vaccine candidate (pGc) elicited a considerable amount of neutralizingantibody responses in the vaccinated mice. The vaccine candidate significantly induced bothantiviral Th1 and B cell activating Th2 immune responses deduced from the cytokineproduction profiles in the vaccinated mice. However, despite the immune responses elicitedpost-immunization, the vaccine failed to confer protection against lethal CCHF virus infection.Conclusion: To the best of our knowledge, this is the first report of a DNA vaccine candidategenerated against CCHF virus based on the glycoprotein Gc. The pGc vaccine candidate exhibitedantigen-specific immunity in IFN/?/?R-/- mice, but was unable to produce a protection upon lethalchallenge with the homologous CCHF virus. Once we comprehensively understand the immunecorrelates of protection, we will be more eligible to significantly improve the efficacy of vaccines.
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23
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1
