Concentration-Dependent Effects of Zinc Sulfate on DU-145 Human Prostate Cancer Cell Line: Oxidative, Apoptotic, Inflammatory, and Morphological Analyzes

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dc.contributor.authorHacıoğlu, Ceyhan
dc.contributor.authorKaçar, Sedat
dc.contributor.authorKar, Fatih
dc.contributor.authorKanbak, Güngör
dc.contributor.authorŞahintürk, Varol
dc.date.accessioned2020-04-30T13:32:15Z
dc.date.available2020-04-30T13:32:15Z
dc.date.issued2019
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractZinc takes part in several of cellular signaling pathways, containing defense against free radicals, apoptosis, and inflammation. However, interaction between zinc and prostate cancer progression is poorly understood. Therefore, zinc treatment in DU-145 human prostate cancer cells was investigated. First, zinc sulfate (ZnSO4) concentrations with antiproliferative effect were determined using MTT assay. Then, ZnSO4-induced oxidative damage was evaluated by malondialdehyde (MDA) levels, glutathione (GSH) levels, total oxidant status (TOS) levels, and total antioxidant status (TAS) levels. Apoptotic effects of ZnSO4 were determined by measuring biochemical and immunohistochemical parameters including caspase 3 (CASP3), cytochrome C (CYC), Bcl-2-associated X protein (Bax), and B cell CLL/lymphoma 2 (Bcl-2) levels. Inflammatory effects of ZnSO4 were investigated by measuring interleukin-6 (IL-6) levels and tumor necrosis factor-alpha (TNF-?) levels. Finally, morphological analysis was performed using hematoxylin-eosin staining. We found that ZnSO4 caused a concentration-dependent increase in oxidative stress, apoptosis, and inflammation pathways. Moreover, there were a number of morphological alterations in treated cells depending on the ZnSO4 concentration. Consequently, our data showed that zinc acts as a regulator of increased oxidative damage and apoptosis through the upregulation of TNF-? and IL-6. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.en_US
dc.identifier.doi10.1007/s12011-019-01879-0en_US
dc.identifier.issn0163-4984
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s12011-019-01879-0
dc.identifier.urihttps://hdl.handle.net/20.500.12684/189
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherHumana Press Inc.en_US
dc.relation.ispartofBiological Trace Element Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosis; DU-145 prostate cancer cells; Inflammation; Oxidative stress; Zinc sulfateen_US
dc.titleConcentration-Dependent Effects of Zinc Sulfate on DU-145 Human Prostate Cancer Cell Line: Oxidative, Apoptotic, Inflammatory, and Morphological Analyzesen_US
dc.typeArticleen_US

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