Assessment of miRNA expression profile and differential expression pattern of target genes in cold-tolerant and cold-sensitive tomato cultivars

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dc.contributor.authorKoç, İbrahim
dc.contributor.authorFiliz, Ertuğrul
dc.contributor.authorTombuloğlu, Hüseyin
dc.date.accessioned2020-04-30T22:39:47Z
dc.date.available2020-04-30T22:39:47Z
dc.date.issued2015
dc.departmentDÜ, Çilimli Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.descriptionFiliz, Ertugrul/0000-0001-9636-6389; Tombuloglu, Huseyin/0000-0001-8546-2658en_US
dc.descriptionWOS: 000360295700006en_US
dc.description.abstractMircroRNAs (miRNAs) are small non-coding RNAs about 21 nt in length. These short transcripts regulate developmental and stress responses in plants. Cold stress is one of the most restraining abiotic factors adversely affecting the plant yield. In the present study, some cold stress-related miRNAs (miR167, miR169, miR172, miR393 and miR397) in tomato (Solanum lycopersicum) were assessed at early time points (0, 1, 4 and 16 h) of cold exposure. Relative expression of miRNAs was measured by stem-loop quantitative reverse transcription polymerase chain reaction. The results showed that miR167, miR169, miR172 and miR393 were activated in the early time points of cold treatment. Especially, miR172 was found to have highest expression level. Furthermore, target genes of selected miRNAs were identified and their expression profiles were assessed between cold-sensitive and cold-tolerant cultivars of tomato. It was found that inferred expression patterns of target genes were differentiated between the cultivars. Analysis of cis-acting elements showed that miRNAs had stress-responsive elements. Meanwhile, since no miR393 sequence is available, putative miR393 sequence and its secondary structure were predicted in tomato. These results may provide a framework for further analysis in terms of understanding the response of miRNAs against cold stress in tomato.en_US
dc.identifier.doi10.1080/13102818.2015.1061447en_US
dc.identifier.endpage860en_US
dc.identifier.issn1310-2818
dc.identifier.issn1314-3530
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage851en_US
dc.identifier.urihttps://doi.org/10.1080/13102818.2015.1061447
dc.identifier.urihttps://hdl.handle.net/20.500.12684/2824
dc.identifier.volume29en_US
dc.identifier.wosWOS:000360295700006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiotechnology & Biotechnological Equipmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcold stressen_US
dc.subjectmiRNAen_US
dc.subjectstem-loop qRT-PCRen_US
dc.subjecttomatoen_US
dc.subjectcis-acting elementen_US
dc.subjecttarget genesen_US
dc.titleAssessment of miRNA expression profile and differential expression pattern of target genes in cold-tolerant and cold-sensitive tomato cultivarsen_US
dc.typeArticleen_US

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