Can Pulsed Electric Fields Be an Alternative for Disinfection in Endodontic Treatment?

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dc.contributor.authorAydin, Zeliha Ugur
dc.contributor.authorErdonmez, Demet
dc.contributor.authorEvrendilek, Gulsun Akdemir
dc.date.accessioned2026-07-01T11:39:26Z
dc.date.available2026-07-01T11:39:26Z
dc.date.issued2025
dc.departmentDüzce Üniversitesi
dc.description.abstractPersistent Enterococcus faecalis infections represent a major cause of endodontic treatment failure, highlighting the need for innovative disinfection strategies beyond conventional irrigation. This in vitro study evaluated the antimicrobial potential of pulsed electric fields (PEF) as a non-thermal and non-chemical adjunctive method for root canal disinfection. Fifty-two extracted mandibular premolars with single canals were standardized to 16 +/- 0.1 mm, inoculated with E. faecalis, and incubated for 28 days to establish mature biofilms. The teeth were subsequently exposed to PEF under varying electrical parameters using sterile water as the irrigant, and bacterial viability and metabolic activity were assessed post-treatment. Confocal laser scanning microscopy (CLSM) was performed to visualize bacterial distribution within dentinal tubules, with particular attention to the apical region, which is most resistant to disinfection. PEF application significantly reduced bacterial viability and metabolic activity compared with baseline (p < 0.05), although complete elimination was not achieved. CLSM images revealed both red-stained non-viable cells, reflecting irreversible electroporation, and green-stained viable cells, indicating reversible electroporation and residual bacterial survival. These findings demonstrate that PEF can inactivate microorganisms through electroporation while maintaining tissue compatibility, and its antimicrobial effect may be enhanced when combined with sodium hypochlorite. Optimization of electrical parameters and evaluation in polymicrobial biofilm models are warranted to establish clinical relevance and support translation into practice.
dc.identifier.doi10.3390/app152011133
dc.identifier.issn2076-3417
dc.identifier.issue20
dc.identifier.orcid0000-0002-5331-2206
dc.identifier.orcid0000-0001-5064-4195
dc.identifier.scopus2-s2.0-105020239287
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/app152011133
dc.identifier.urihttps://hdl.handle.net/20.500.12684/23270
dc.identifier.volume15
dc.identifier.wosWOS:001604425300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofApplied Sciences-Basel
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260623
dc.subjectElectroporation
dc.subjectEnterococcus Faecalis
dc.subjectRoot Canal Therapy
dc.subjectEndodontics
dc.subjectElectric Stimulation
dc.subjectAnti-Bacterial Agents
dc.titleCan Pulsed Electric Fields Be an Alternative for Disinfection in Endodontic Treatment?
dc.typeArticle

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