The Role of Estrogen Receptors in the Therapeutic Effect of Oxyresveratrol, Phytoestrogen, During, and After Oxidative Stress
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pleiades Publishing Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: Oxidative stress plays a significant role in the development and progression of various neurodegenerative disorders, including aging, atherosclerosis, cancer, ischemia reperfusion, inflammation, rheumatoid arthritis, liver diseases. Additionally, it is implicated in retinal diseases (RD) such as glaucoma, diabetic retinopathy (DR), retinal vein occlusion (RVO), and age-related macular degeneration (AMD). In our study, we studied the therapeutic effect of oxyresveratrol, against H2O2-induced oxidative damage. Additionally, we investigated the role of estrogen receptors in the observed effects. Methods: Oxidative stress was induced by H2O2 in ARPE-19 cells. Oxyresveratrol was applied at seven different concentrations during and after oxidative stress. Besides, estrogen receptor inhibitors were applied 1 h before the treatment to investigate the role of estrogen receptors. Changes in cell viability were assessed using XTT. To investigate the effectiveness of oxyresveratrol at the molecular level, cell death detection, Caspase-3, and TOS measurement kits were employed. Results and Discussion: Our findings indicated that oxyresveratrol at concentrations of 10 and 100 mu M reduced cell damage in ARPE-19 cells during and after-induced oxidative stress. Additionally, the therapeutic effect of oxyresveratrol in ARPE-19 cells during oxidative stress formation appeared to be dependent on estrogen receptors alpha and beta, while the therapeutic effect after oxidative stress seemed to be GPER1-dependent. Furthermore, oxyresveratrol suppressed apoptosis in ARPE-19 cells under oxidative stress, reducing cell death, and both during and after oxidative stress, oxyresveratrol application decreased TOS levels. Although the antioxidant, anti-inflammatory, and neuroprotective effects of oxyresveratrol are well known, this is the first study to investigate its therapeutic effects on H2O2 induced oxidative stress in ARPE-19 cells and the involvement of estrogen receptors in these effects. Conclusions: We believe that oxyresveratrol may be an alternative therapy in the prevention and treatment of retinal diseases.
Açıklama
Anahtar Kelimeler
ARPE-19, phytoestrogen, oxyresveratrol, oxidative stress, estrogen receptors
Kaynak
Russian Journal of Bioorganic Chemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q4
Cilt
50
Sayı
6
