Ensuring traceability of organophosphate pesticides (OPs) through enzyme immobilized spheres

Basit Öğe Kaydı
dc.authoridHASANOGLU OZKAN, ELVAN/0000-0001-7338-4015
dc.authorwosidOZKAN, Elvan HASANOGLU/AAJ-2225-2021
dc.contributor.authorHasanoglu ozkan, Elvan
dc.contributor.authorKurnaz Yetim, Nurdan
dc.contributor.authorNartop, Dilek
dc.contributor.authorSari, Nursen
dc.date.accessioned2021-12-01T18:49:31Z
dc.date.available2021-12-01T18:49:31Z
dc.date.issued2021
dc.department[Belirlenecek]en_US
dc.description.abstractCurrently, pesticide residues increasingly lead to health problems. Acetylcholinesterase enzyme (AChE) plays a role in detoxification processes due to their ability to scavenge organophosphates. Thus, this enzyme has been selected for the detection of pesticides. Herein, this study describes the preparation and application of appropriate new immobilized spheres that could be used for the detection of organophosphorus pesticide residues. That is a simple colorimetric enzymatic assay for the practical discovery of N-(Mercaptomethyl) phthalimide S-(O, O-dimethylphosphorodithioate) from organophosphorus pesticides (OPs) widely used in the treatment of sugar beet, apple, nut, corn, and tobacco. The process is based on the immobilization of acetylcholinesterase onto tryptophan FMPS-Trp, (FMPS-Trp)Pd(II), and (FMPS-Trp)Pt(II) functionalized spheres. Spectroscopic and microscopic techniques were used in the characterization of the spheres where scanning electron microscopy (SEM) is an essential tool. The (FMPS-Trp)Pt(II)-AChE showed a good affinity to acetylthiocholine chloride (ATCl) and was found to have the ability to catalyze the hydrolysis of ATCl with an apparent Michaelis-Menten constant value of 288 mM. The developed colorimetric process showed good qualitative analytical performance for Phosmet (OPs) detection as low as 10(-7) M. [GRAPHICS] .en_US
dc.description.sponsorshipGazi University Research FundGazi University [05/2014-02]en_US
dc.description.sponsorshipThis work was supported by the Gazi University Research Fund (05/2014-02).en_US
dc.identifier.doi10.1007/s13738-020-02147-y
dc.identifier.endpage1759en_US
dc.identifier.issn1735-207X
dc.identifier.issn1735-2428
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-85099954280en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1749en_US
dc.identifier.urihttps://doi.org/10.1007/s13738-020-02147-y
dc.identifier.urihttps://hdl.handle.net/20.500.12684/10734
dc.identifier.volume18en_US
dc.identifier.wosWOS:000612292300001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal Of The Iranian Chemical Societyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcetylcholinesterase enzymeen_US
dc.subjectAcetylthiocholine chlorideen_US
dc.subjectFunctionalized spheresen_US
dc.subjectPhosmeten_US
dc.titleEnsuring traceability of organophosphate pesticides (OPs) through enzyme immobilized spheresen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
10734.pdf
Boyut:
1.76 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu