Determination of some phenolics and fatty acid compounds from an endemic Cephalaria species in Anatolia along with antioxidant and anti-inflammatory activity

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dc.authoridKaraaslan, Melek/0000-0002-9910-2909en_US
dc.authorscopusid50161317500en_US
dc.authorscopusid58243109300en_US
dc.authorscopusid58241997700en_US
dc.authorscopusid57224207262en_US
dc.authorscopusid24170635400en_US
dc.authorscopusid57202648342en_US
dc.authorwosidAKSOY, NECMI/G-3870-2011en_US
dc.authorwosidKaraaslan, Melek/AAG-9349-2020en_US
dc.contributor.authorErgene, Burcin
dc.contributor.authorKaraaslan, Melek
dc.contributor.authorOzler, Kamuran Ileri
dc.contributor.authorSarialtin, Sezen Yilmaz
dc.contributor.authorAksoy, Necmi
dc.contributor.authorIscan, Gulcin Saltan
dc.date.accessioned2024-08-23T16:03:40Z
dc.date.available2024-08-23T16:03:40Z
dc.date.issued2023en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractIn this study, HPLC analyses of total extract prepared with aqueous methanol, butanol fraction, and water fraction of Cephalaria duzceensis N.Aksoy & Gokturk (Caprifoliaceae), which is a local endemic species in Turkey, were conducted and contents of chlorogenic acid were calculated. The total antioxidant capacity, ABTS and DPPH free radical scavenging activity of the total extract were determined to evaluate its antioxidant activity. Assessment of anti-inflammatory activity was done using human red blood cell membrane stabilization and protein denaturation inhibition activity tests In vitro, whereas carrageenan-induced hind paw edema test In vivo. Moreover, the fatty acid composition inside the fixed oil extracted from C. duzceensis seeds was determined by GC -MS. As the result of GC -MS analysis, linoleic and oleic acids were found to be the predominant fatty acids in the fixed oil. The total phenolic contents of the total extract and butanol fraction were determined as 63.4029 and 95.0131 mg GAE/g dry extract, respectively. The chlorogenic acid amounts of the total extract, butanol, and water fractions were calculated as 3.7494%, 3.5335%, and 1.2354% by HPLC analysis. IC50 values of the total extract were calculated as 45.1385 mu g/ml against ABTS center dot+ and 28.6407 mu g/ml against DPPH center dot radicals and the total antioxidant capacity was 45.84 mg ascorbic acid equivalent/g dry extract. IC50 values of the total extract were calculated as 1.4084 mg/ml for human red blood cell membrane stabilization and 1.8601 mg/ml for protein denaturation inhibition method showing its moderate activity. In vivo tests revealed that total extract caused almost as much inhibition on edema as 10 mg/kg diclofenac sodium.en_US
dc.identifier.doi10.30848/PJB2023-4(28)
dc.identifier.endpage1427en_US
dc.identifier.issn0556-3321
dc.identifier.issn2070-3368
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85158890757en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1419en_US
dc.identifier.urihttps://doi.org/10.30848/PJB2023-4(28)
dc.identifier.urihttps://hdl.handle.net/20.500.12684/13855
dc.identifier.volume55en_US
dc.identifier.wosWOS:000972097200023en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPakistan Botanical Socen_US
dc.relation.ispartofPakistan Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnti-inflammatoryen_US
dc.subjectAntioxidanten_US
dc.subjectCephalariaen_US
dc.subjectFatty aciden_US
dc.subjectGC-MSen_US
dc.subjectHPLCen_US
dc.subjectTriterpenoid Saponinsen_US
dc.subjectMedicinal-Plantsen_US
dc.subjectCaprifoliaceaeen_US
dc.titleDetermination of some phenolics and fatty acid compounds from an endemic Cephalaria species in Anatolia along with antioxidant and anti-inflammatory activityen_US
dc.typeArticleen_US

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