A Bioinformatics-Based Approach for Designing Primer Sets in Determination of Meat Specificity

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dc.contributor.authorEren, Ömer
dc.contributor.authorMilli, Nursel Söylemez
dc.contributor.authorÜnlü, Ercan
dc.contributor.authorParlak, İsmail Hakkı
dc.contributor.authorMilli, Mehmet
dc.date.accessioned2023-04-10T20:27:53Z
dc.date.available2023-04-10T20:27:53Z
dc.date.issued2021
dc.departmentRektörlük, Rektörlüğe Bağlı Birimler, Düzce Üniversitesi Dergilerien_US
dc.description.abstractPolymerase chain reaction (PCR) and its derivatives are one of the most widely used DNA-based methods in species determination studies in meat and meat products. Chromosomal or mitochondrial genes of the species can be targeted in PCR-based analyzes used in species detection studies. Many researchers are able to realize oligonucleotide differences between species through online alignment programs on mitochondrial DNA. Using chromosomal DNA would provide more concise results in quantification studies. However, determining the marker regions for genomic DNA is challenging due to the large size of the chromosomes. Bioinformatics approaches are available for selected applications. However, using those approaches requires intensive knowledge of computer science, molecular biology, and bioinformatics in addition to high computational power. In this study, a pipeline is presented that will provide a user-friendly approach to be adopted by facilities where contamination analyzes are routinely performed.en_US
dc.identifier.doi10.29130/dubited.898519
dc.identifier.endpage1675en_US
dc.identifier.issn2148-2446
dc.identifier.issue5en_US
dc.identifier.startpage1669en_US
dc.identifier.trdizinid497720en_US
dc.identifier.urihttp://doi.org/10.29130/dubited.898519
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/497720
dc.identifier.urihttps://hdl.handle.net/20.500.12684/11784
dc.identifier.volume9en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofDüzce Üniversitesi Bilim ve Teknoloji Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleA Bioinformatics-Based Approach for Designing Primer Sets in Determination of Meat Specificityen_US
dc.typeArticleen_US

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