What is the restoring role of chrysin in alpha amanitin toxicity

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dc.contributor.authorBakırcı, Sinan
dc.contributor.authorBayram, Recep
dc.contributor.authorKaya, Ertuğrul
dc.contributor.authorYaykaşlı, Kürşat Oğuz
dc.contributor.authorYılmaz, İsmail
dc.date.accessioned2020-04-30T13:34:05Z
dc.date.available2020-04-30T13:34:05Z
dc.date.issued2015
dc.departmentDÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractAim: To explore whether chrysin has any protective effect against the deadly effect of alpha amanitin on hepatocytes that is one of the major toxins in the structure of Amanita phalloides, which is considered the most important mushroom responsible for fatal mushroom poisonings. Materials and methods: For this study, alpha amanitin was purified from A phalloides mushroom using the preparative HPLC (high performance liquid chromatography) method as described in the literature. Four hours after administering alpha amanitin in a concentration of 10 ?g/mL on the cells in a hepatocyte cell line (C3A), silibinin and chrysin were administered in various concentrations. The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] test was used to determine cell viability. Results: The alpha amanitin was obtained in high purity from the mushrooms and was found to decrease cell viability down to a level of 63% after 48 hours due to its toxic effect on the liver cells in the cell culture. In the groups given silibinin and chrysin, both substances were seen to reduce the toxicity caused by alpha amanitin. Conclusion: Chrysin may play a role in decreasing the tissue damage caused by toxins and lowering the mortality rates in fatal mushroom poisonings associated with alpha amanitin.en_US
dc.identifier.endpage1185en_US
dc.identifier.issn0393-6384
dc.identifier.issue6en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1181en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12684/801
dc.identifier.volume31en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherA. CARBONE Editoreen_US
dc.relation.ispartofActa Medica Mediterraneaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha amanitin; C3A human hepatocyte cell line; Chrysin; Mushroom poisoning; Protectionen_US
dc.titleWhat is the restoring role of chrysin in alpha amanitin toxicityen_US
dc.typeArticleen_US

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