Screening of medicinal plant extracts as novel DNA gyrase inhibitors

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dc.authoridSIMAY DEMIR, YAPRAK DILBER/0000-0002-8248-0268en_US
dc.authorscopusid56926708500en_US
dc.authorscopusid56230588400en_US
dc.authorscopusid23570520200en_US
dc.authorscopusid58286348400en_US
dc.authorscopusid59157742600en_US
dc.contributor.authorOyardi, O.
dc.contributor.authorOzcelik, B.
dc.contributor.authorÇalışkan, U. Koca
dc.contributor.authorDemir, Y. D. Simay
dc.contributor.authorArk, M.
dc.date.accessioned2024-08-23T16:03:22Z
dc.date.available2024-08-23T16:03:22Z
dc.date.issued2023en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractBioactivities of a number of medicinal plants; Alkanna tinctoria (L.) Tausch, Alnus glutinosa (L.) Gaertn., Calamintha nepeta Willk. and C. nepeta, Centaurea iberica Trevir. ex Spreng., Citrus paradisi Macfad., C. paradisi, Citrus sinensis (L.) spicata L., T. spicata (n: 20), which are used for the prevention and treatment of diverse diseases, were investigated. The antimicrobial activities of extracts were evaluated using broth microdilution assay. The cytotoxicities of extracts were investigated on HeLa cell line by MTT assay. Statistical analysis was performed using GraphPad Prism (5.0). The effects of the extracts, which have the highest antimicrobial activity, on the Escherichia coli and Staphylococcus aureus DNA gyrase gene expression were determined by using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The MICs (& mu;g/ml) of extracts were determined as 32-64, 2-128, 8-128,1-128, 4-128 against Gram-positive, Gram-negative bacteria, yeasts, dermatophytes, and Mycobacterium spp., respectively. No cytotoxicity has been observed in plant extracts tested. DNA gyrase activity was determined for T. capitatus-SFE (128 & mu;g/mL) and L. nobilis-Hx (128 & mu;g/mL) extracts according to the inhibition of DNA gyrase gene expression. Overall, T. capitatus-SFE and L. nobilis-Hx are good candidates for further antimicrobial studies.en_US
dc.identifier.doi10.56042/ijtk.v22i1.45566
dc.identifier.endpage219en_US
dc.identifier.issn0972-5938
dc.identifier.issn0975-1068
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85160034784en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage211en_US
dc.identifier.urihttps://doi.org/10.56042/ijtk.v22i1.45566
dc.identifier.urihttps://hdl.handle.net/20.500.12684/13709
dc.identifier.volume22en_US
dc.identifier.wosWOS:001022147600025en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.relation.ispartofIndian Journal of Traditional Knowledgeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntimicrobial activityen_US
dc.subjectAnti-dermatophytic activityen_US
dc.subjectAnti-Mycobacterium activityen_US
dc.subjectAromatic medicinal plantsen_US
dc.subjectCytotoxicityen_US
dc.subjectDNA gyrase inhibitionen_US
dc.subjectIn-Vitroen_US
dc.subjectThymus-Capitatusen_US
dc.subjectAntioxidanten_US
dc.subjectL.en_US
dc.subjectFlavonoidsen_US
dc.subjectOilsen_US
dc.titleScreening of medicinal plant extracts as novel DNA gyrase inhibitorsen_US
dc.typeArticleen_US

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