Chemical composition, antioxidant, antimicrobial and anticancer activities of endemic Cephalaria tuteliana

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dc.authoridBOZYEL, Mustafa Eray/0000-0001-5556-4880en_US
dc.authoridSenturk, Fatih/0000-0002-2436-3362en_US
dc.authorscopusid56156721800en_US
dc.authorscopusid25229718700en_US
dc.authorscopusid57223847596en_US
dc.authorscopusid57205553123en_US
dc.authorscopusid55552934100en_US
dc.authorscopusid56032304900en_US
dc.authorwosidBOZYEL, Mustafa Eray/W-4530-2019en_US
dc.authorwosidSenturk, Fatih/AEI-3613-2022en_US
dc.contributor.authorKiliccioglu, Ilker
dc.contributor.authorDulger, Gorkem
dc.contributor.authorSenturk, Fatih
dc.contributor.authorBozyel, Mustafa Eray
dc.contributor.authorCanli, Kerem
dc.contributor.authorDulger, Basaran
dc.date.accessioned2024-08-23T16:04:33Z
dc.date.available2024-08-23T16:04:33Z
dc.date.issued2024en_US
dc.departmentDüzce Üniversitesien_US
dc.description.abstractIntroduction: The genus Cephalaria, belonging to the Caprifoliaceae family, is a rich source of secondary metabolites, including mainly saponins which display a variety of biological activities, such as antimicrobial, antioxidant, and anticancerous effects. This study was carried out to observe the biochemical composition and to evaluate the biological activities of Cephalaria tuteliana Kus & Gokturk. Methods: The composition of endemic C. tuteliana was determined through gas chromatography-mass spectrometry (GC-MS) studies. Antimicrobial effect were determined by the disk diffusion and dilution methods for test bacteria and test fungi. The antioxidant potential of the ethanol extract was also investigated. Cell proliferation and apoptosis analysis were investigated by WST-1 and ELISA methods. The migration level of PC-3 prostate cancer cells after plant extract application was examined by wound healing assay. Also, molecular docking experiments were performed to investigate the anticancer activity of the major component found in the C.tuteliana extract. Results: GC-MS analysis showed that the predominant compounds were Phenol, 2,20-methylenebis [6-(1,1dimethyl ethyl)-4-methyl- (23.54 %), 9,12,15-octadecatrien-1-ol, (Z,Z,Z)-(6.06 %) and n-Hexadecanoic acid (3.03 %). The ethanolic extract of C. tuteliana displayed moderate antimicrobial activity against a spectrum of tested microorganisms. Also, it exhibited significant antiproliferative effects on PC-3 human prostate cancer cells while having no harmful effects on healthy control HUVEC cells. Furthermore, enhanced expressions of Caspase-3, 8, and 9 were observed in PC-3 cells after plant extract treatment. Also, cell migration level was notably reduced, especially at a dose of 400 mg/mL in PC-3 cells. The DPPH free radical scavenging activity of ethanolic extracts of C. tuteliana showed higher activity than ascorbic acid. Molecular docking experiments showed potential binding of the major aromatic compound in C.tuteliana extract to human androgen receptor 1E3G protein. Conclusion: These findings highlight the potential of C. tuteliana as a valuable natural resource and pave the way for further in vivo prostate cancer investigations. (c) 2024 SAAB. Published by Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.sajb.2024.04.038
dc.identifier.endpage241en_US
dc.identifier.issn0254-6299
dc.identifier.issn1727-9321
dc.identifier.scopus2-s2.0-85191349892en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage231en_US
dc.identifier.urihttps://doi.org/10.1016/j.sajb.2024.04.038
dc.identifier.urihttps://hdl.handle.net/20.500.12684/14265
dc.identifier.volume169en_US
dc.identifier.wosWOS:001235412500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofSouth African Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCephalaria tutelianaen_US
dc.subjectDPPHen_US
dc.subjectGC-MSen_US
dc.subjectAntimicrobialen_US
dc.subjectProstate cancer cellsen_US
dc.subjectTriterpenoid Saponinsen_US
dc.subjectProstate-Canceren_US
dc.subjectGlycosidesen_US
dc.subjectPotenten_US
dc.titleChemical composition, antioxidant, antimicrobial and anticancer activities of endemic Cephalaria tutelianaen_US
dc.typeArticleen_US

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