Biological Activity of Natural Formulation with Propolis, Lavender and Thyme Oil on Candida Species
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Candida strains have been frequently associated with nosocomial infections in recent years. On the other hand, the public prefers natural resources rather than synthetic materials. Therefore, it is essential to research the combined effects of natural products. This study aimed to investigate the antifungal and antibiofilm effectiveness of essential oils and propolis-containing samples on Candida species and the toxicity of samples. Materials-Methods: Three different samples were prepared with propolis, lavender oil, and thyme oil. Gas Chromatography-mass Spectrometry (GC/MS) GC/MS was used to determine organic compounds in samples. The antifungal effects of a natural product mix were tested against C. albicans, C. krusei, and C. parapsilosis by disk diffusion method. MIC and MFC tests of the mixture were performed against a standard such as Nystatin. Antibiofilm tests were performed with the microplate system, taking into account the detected concentrations, and finally, the cytotoxicity test was investigated in the L929 cell line with the WST-1 kit. Results: C. albicans was determined as the most sensitive species (MIC50: 90 ppm, MIC90: 750 ppm MFC: 3120 ppm). In the antibiofilm tests, it was determined that the sample was more effective on C. albicans, and it inhibited biofilm formation. Also, it was determined that the IC50 value was 5052 ppm. Conclusion: The natural product mixture, which has a scientifically proven antifungal effect, has a feature that has high added value and contributes to the economy. However, of course, it has to investigate by further studies. The findings of this study may lead to new therapeutic approaches with standardized natural products in the future.
Açıklama
Anahtar Kelimeler
Kaynak
International journal of traditional and complementary medicine research (Online)
WoS Q Değeri
Scopus Q Değeri
Cilt
4
Sayı
2
