The measurement of alpha amanitin levels using hplc method in Amanita phalloides from Düzce province

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dc.contributor.authorKaya, Ertuğrul
dc.contributor.authorKarahan, Selim
dc.contributor.authorHancı, Mustafa
dc.contributor.authorYaykaşlı, Kürşat Oğuz
dc.contributor.authorSarıtaş, Ayhan
dc.contributor.authorBayram, Recep
dc.contributor.authorArslan, Seyfullah Oktay
dc.date.accessioned2020-04-30T13:33:45Z
dc.date.available2020-04-30T13:33:45Z
dc.date.issued2012
dc.departmentDÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractAim: The aim of this study is to measure the level of alpha amanitin toxin using HPLC method from Amanita phalloides mushroom collected in the province of Düzce in 2010. Method: One of the mushrooms as a whole body. The other one was extracted after seperated into parts. The measurement was done by HPLC using 303 nm UV wavelength and 250x4,6 mm C18 5 ?m particle included column. Ammonium acetate+methanol+acetonitrile (80+10+10, v/v/v) was used as a mobile phase, and the flow rate was set 1 ml/min. The results were given as a toxin quantity in 1 g dry mushroom. Results: The amount of alpha amanitin was measured as 4,806 mg (±0,033) in the whole body, 3,522 mg (±0,024) in the cap, 5,318 mg (±0,056) in the lamellar, 0,903 mg (±0,004) in the ring, 2,577 mg (±0,037) in the stipe, 0,698 mg (±0,008) in the volva. Conclusion: The level of alpha amanitin in Amanita phalloides from Duzce Province is differ from different countries. Higher and lower levels of toxin than our data obtained investigations are present in the literature. The reason of this differences might be several factors like extraction methods, analysis methods and environmental conditions. © 2012 Düzce Medical Journal.en_US
dc.identifier.endpage17en_US
dc.identifier.issn1307671X
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage15en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12684/723
dc.identifier.volume14en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherDuzce University Medical Schoolen_US
dc.relation.ispartofDuzce Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha amanitin; Amanita phalloides; HPLCen_US
dc.titleThe measurement of alpha amanitin levels using hplc method in Amanita phalloides from Düzce provinceen_US
dc.title.alternativeDüzce yöresinde yetişen amanita phalloides mantarındaki alfa amanitin düzeyinin hplc yöntemiyle ölçümüen_US
dc.typeArticleen_US

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