Sıçanlarda akut ve kronik uygulanan harmalinin penisilin ile oluşturulmuş epileptiform aktivite üzerine etkisi
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hayat Sağlık ve Sosyal Hizmetler Vakfı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Harmalin (HR), modern tıbbı desteklemek amacıyla deneysel çalışmaların yapıldığı Banisteriopsis caapi ve Peganum harmala bitkilerinden elde edilen, monoamin oksidaz inhibitörü (MAOi) ve antioksidan bir alkaloiddir. Bu çalışmada kısa süreli ve uzun süreli HR tedavisinin sıçanlarda penisilin G ile indüklenen epileptik model üzerindeki elektrofizyolojik etkisi incelenmiştir. Yöntemler: Seksen dört yetişkin erkek Wistar sıçan rastgele iki gruba ayrıldı. Akut gruba tek doz/gün HR, kronik gruba tekrarlayan doz/gün HR verildi. Her grup ayrıca HR (10, 50 ve100 mg/kg) dozuna göre altı alt gruba ayrıldı. İntrakortikal penisilin uygulamasıyla deney gruplarında epileptiform aktivite (EA) tetiklendi. Elektrofizyolojik veriler, elektrokortikografi (ECoG) kullanılarak izlendi ve analiz edildi. HR’nin serbest radikal temizleyici etkilerini değerlendirmek için süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx) ve glutatyon redüktazın (GR) serum seviyeleri Enzim Bağlı İmmüno-Sorbent testi (ELISA) yöntemi kullanılarak ölçüldü. EA dalgalarının latensi, frekansı ve amplitüdü ile serum antioksidan belirteç düzeyleri istatistiksel olarak analiz edildi. Bulgular: Sham grubunda EA görülmedi. Ancak sonuçlar hem akut hem de kronik HR tedavisinin nöbet eşiğini doza bağlı olarak arttırdığını gösterdi (p
Aim: Harmaline (HR) is a monoamine oxidase inhibitor (MAOi) and antioxidant alkaloid obtained from Banisteriopsis caapi and Peganum harmala, where experimental studies have been conducted to support modern medicine. The electrophysiological impact of short-term and long-term HR treatment on the penicillin G-induced epileptic model in rats was examined in this study. Methods: Eighty-four adult male Wistar rats were randomly assigned to two groups: one received a single dose/day of HR, and the other received repeated doses/days of HR. Each group was further divided into six subgroups based on the dose of HR (10, 50, and 100 mg/kg). Epileptiform activity (EA) was triggered in the experimental groups with intracortical penicillin administration. Electrophysiological data were collected and analyzed using electrocorticography (ECoG). The serum levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were measured using the Enzyme-Linked Immuno Sorbent Assay (ELISA) method to assess the free radical scavenger effects of HR. The latency, frequency, and amplitude of EA waves and serum antioxidant marker levels were analyzed statistically. Results: There was no observed EA in the sham group. Nevertheless, the results showed that both acute and chronic HR treatment increased the seizure threshold dose-dependently (p
Aim: Harmaline (HR) is a monoamine oxidase inhibitor (MAOi) and antioxidant alkaloid obtained from Banisteriopsis caapi and Peganum harmala, where experimental studies have been conducted to support modern medicine. The electrophysiological impact of short-term and long-term HR treatment on the penicillin G-induced epileptic model in rats was examined in this study. Methods: Eighty-four adult male Wistar rats were randomly assigned to two groups: one received a single dose/day of HR, and the other received repeated doses/days of HR. Each group was further divided into six subgroups based on the dose of HR (10, 50, and 100 mg/kg). Epileptiform activity (EA) was triggered in the experimental groups with intracortical penicillin administration. Electrophysiological data were collected and analyzed using electrocorticography (ECoG). The serum levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were measured using the Enzyme-Linked Immuno Sorbent Assay (ELISA) method to assess the free radical scavenger effects of HR. The latency, frequency, and amplitude of EA waves and serum antioxidant marker levels were analyzed statistically. Results: There was no observed EA in the sham group. Nevertheless, the results showed that both acute and chronic HR treatment increased the seizure threshold dose-dependently (p
Açıklama
Anahtar Kelimeler
Antioksidanlar|elektrokortikografi|epilepsi|harmalin|monoamin oksidaz|Antioxidants|electrocorticography|epilepsy|harmaline|monoamine oxidase
Kaynak
Anatolian Clinic the Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
29
Sayı
3
