Characterization of a Thermophilic Lipase from Bacillus licheniformis Ht7 Isolated from Hayran Thermal Springs in Giresun

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dc.contributor.authorUğraş, Serpil
dc.date.accessioned2020-04-30T22:40:43Z
dc.date.available2020-04-30T22:40:43Z
dc.date.issued2017
dc.departmentDÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.descriptionWOS: 000395934100018en_US
dc.description.abstractIn this study five thermotolerant bacteria were isolated from water and soil of the Hayran thermal springs in Giresun. These bacteria were identified as four Bacillus licheniformis (Bl-Ht1, Bl-Ht2, Bl-Ht5, and Bl-Ht7) and a Bacillus subtilis (Bs-Ht9) based on morphological, physiological, and biochemical characteristics, as well as phylogenetic analysis using 16S rDNA sequences. The strain Bl-Ht7 was exhibited the highest lipase activity, and the specific lipase activity was calculated as 15.916 U g(-1). The maximum enzyme activity was exhibited at 90 degrees C and pH 9.0. The molecular weight was determined as approximate 22 kDa and it was confirmed using the zymogram analysis. The lipase activity was enhanced by the application of Ca2+, C (2+), Zn2+ ions, and some organic solvents but no important differences with the other applicatio ns. The optimal production time and suitable carbon source for the production of lipase were found to 24 h, and olive oil, respectively. The findings show that the temperature of the maximum enzyme activity is higher than the other known Bacillus lipases. The enzyme exhibited tolerance to high temperature, organic solvents, and metal ions, so it is attractive for industrial applications.en_US
dc.description.sponsorshipGiresun UniversityGiresun University [FEN-BAP-A-160512-39]en_US
dc.description.sponsorshipThis work was supported by Giresun University grant (Project Code FEN-BAP-A-160512-39). The authors also wish to thank Dr. Hatice KATI (Giresun University, Turkey) for helping with the enzyme analysis.en_US
dc.identifier.endpage12306en_US
dc.identifier.issn1224-5984
dc.identifier.issue1en_US
dc.identifier.startpage12297en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12684/3043
dc.identifier.volume22en_US
dc.identifier.wosWOS:000395934100018en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherArs Docendien_US
dc.relation.ispartofRomanian Biotechnological Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus licheniformisen_US
dc.subjectthermal springsen_US
dc.subjectlipaseen_US
dc.subjectzymogram analysisen_US
dc.titleCharacterization of a Thermophilic Lipase from Bacillus licheniformis Ht7 Isolated from Hayran Thermal Springs in Giresunen_US
dc.typeArticleen_US

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