In vitro stability of pregnancy-associated plasma protein A
Küçük Resim Yok
Tarih
2005
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Amaç: PAPP-A gebelik süresince trofoblastlardan yüksek konsantrasyonda salınır ve gebeliğin 11.-14. haftalarında Down sendromu ve diğer kromozomal anomalilerin taranmasında kullanılmaktadır. Bu çalışmada PAPP-A’nın in vitro stabilitesini araştırdık. Materyal ve Metot: Kan örnekleri gönüllü gebe kadınlardan alınarak iki gruba ayrıldı. Birinci gruptaki örnekler 2 ilâ 8C’de 7 gün ve ikinci gruptakiler –20C’de 28 gün boyunca bekletildi. PAPP-A ilk grupta her gün, ikinci grupta ise haftada bir kez ölçüldü. Sonuçlar: Gruplarda yapılan ölçümlerde istatistiksel olarak anlamlı bir fark bulunmadı (p0.05). Tartışma: Serum PAPP-A’nın 2 ilâ 8C’de en az 7 gün ve –20C’de ise en az 28 güne kadar stabilitesini koruduğunu ve normal laboratuvar koşullarında bir aya kadar saklanabileceğini düşünmekteyiz.
Objective: Pregnancy-associated plasma protein A is produced in high concentration by trophoblasts during pregnancy and maternal serum assessment between 11 and 14 weeks of gestation has significant utility in screening for Down syndrome and other chromosomal anomalies. We aimed to investigate the in vitro stability of pregnancy-associated plasma protein A. Materials and Methods: Blood samples were collected from volunteer pregnant women and divided into two groups. The first and second groups were stored at 2 to 8°C for 7 days and at –20°C for 28 days, respectively. Pregnancy-associated plasma protein A was determined each day in the first group and each week in the second group. Results: There was no statistically significant difference between measurements in each group (p>0.05). Conclusion: We concluded that serum pregnancy-associated plasma protein A is stable for at least 7 days at 2 to8°C and 28 days at –20°C, and could be preserved for up to one month in average laboratory conditions.
Objective: Pregnancy-associated plasma protein A is produced in high concentration by trophoblasts during pregnancy and maternal serum assessment between 11 and 14 weeks of gestation has significant utility in screening for Down syndrome and other chromosomal anomalies. We aimed to investigate the in vitro stability of pregnancy-associated plasma protein A. Materials and Methods: Blood samples were collected from volunteer pregnant women and divided into two groups. The first and second groups were stored at 2 to 8°C for 7 days and at –20°C for 28 days, respectively. Pregnancy-associated plasma protein A was determined each day in the first group and each week in the second group. Results: There was no statistically significant difference between measurements in each group (p>0.05). Conclusion: We concluded that serum pregnancy-associated plasma protein A is stable for at least 7 days at 2 to8°C and 28 days at –20°C, and could be preserved for up to one month in average laboratory conditions.
Açıklama
Anahtar Kelimeler
Kadın Hastalıkları ve Doğum
Kaynak
Journal of the Turkish-German Gynecological Association
WoS Q Değeri
Scopus Q Değeri
Cilt
6
Sayı
3
